- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-427 品牌系列 Upstate
商名 - Upstate
描述 Anti-Phosphotyrosine Antibody 背景信息 The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. 产品信息 格式 Affinity Purified 演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Phosphotyrosine Antibody detects level of Phosphotyrosine & has been published & validated for use in IP & WB. 主要应用 - Western Blotting
应用说明 Western Blotting Analysis: A 1:500 dilution from a representative lot detected Phosphotyrosine in 10 µg of EGF treated A431 with Lambda Phosphatase tissue lysate.
Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Phosphotyrosine in 0.5 mg of EGF treated A431 cell lysate生物信息 免疫原品种 In order to produce broad spectrum polyclonal phosphotyrosine antibodies, rabbits were immunized with three phosphorylated immunogens: (1) phosphotyrosine covalently linked to KLH; (2) the c-Src carboxyl terminal regulatory phosphopeptide (TS-T-E-P-Q-pY-Q-P-G-E-N-L; Catalog # 12-218) covalently linked to KLH, and; (3) a phosphopeptide associated with high tyrosine kinase activity in human lymphocytes (R-R-L-I-E-D-A-E-pY-A-A-R-G; Catalog # 12-217) covalently linked to KLH. Both of the phosphopeptide haptens serve as strong substrates for tyrosine phosphatases and are part of the two colorimetric tyrosine phosphatase kits provided by Upstate, Inc. (Catalog # 17-125, 17-126). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The immunoreactivity of the antibody is totally inhibited by the use of 100 mM phenyl phosphate, a phosphotyrosine analog. The phosphotyrosine antibody is purified by immunoaffinity chromatography using either a dual phospho-peptide gel or a BSA-phosphotyrosine gel. All of the phosphotyrosine immunoreactivity present in the antisera is immunoadsorbed whether the antibody is purified by either gel indicating that the antibody is not sequence-specific but specific for phosphotyrosine residues. 品种反应性 Human Species Reactivity Note Human. Predicted to react with All based on 100% sequence homology. 抗体类型 Polyclonal Antibody 修改 - Phosphorylation
纯化方法 Affinity purified 产品使用声明 质量保证 Evaluated by Western Blotting in EGF treated A431 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Phosphotyrosine in 10 µg of EGF treated A431 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,487929,InSolution™ Nocodazole - CAS 31430-18-9 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,AB4250,Anti-CXC Chemokine Receptor 3 Antibody, CT domain
相关产品
merck millipore,默克密理博,400062,Anti-Glucose Transporter-3 Rabbit pAb
merck millipore,默克密理博,ABE604,Anti-Bonzo Antibody
merck millipore,默克密理博,ABD10,Anti-MSX1 (Msh homeobox 1-like protein) Antibody
merck millipore,默克密理博,SF-1383,AR; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,487929,InSolution™ Nocodazole - CAS 31430-18-9 - Calbiochem
merck millipore,默克密理博,AB4250,Anti-CXC Chemokine Receptor 3 Antibody, CT domain
merck millipore,默克密理博,382129,HIV Reverse Transcriptase, Recombinant, E. coli
merck millipore,默克密理博,ECM551,QCM™ Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric
merck millipore,默克密理博,MAB1273C3,Anti-Mitochondria Antibody, clone 113-1, Cy3 Conjugate
merck millipore,默克密理博,PC53,Anti-Glutathione S-Transferase (Ab-1) Rabbit pAb