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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ht, H WB, FC, ICC M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB4120 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MDR1 Antibody, clone JSB-1 Alternate Names - Pgp
- P-glycoprotein
- CD243
- p170
产品信息 格式 Culture Supernatant 控制 - MDR cells
演示 UnPurified mouse culture supernatant containing 0.7% BSA and 0.1% sodium azide as a preservative. 应用 应用 Anti-MDR1 Antibody, clone JSB-1 detects level of MDR1 & has been published & validated for use in FC, IC, IH(P) & WB. 主要应用 - Western Blotting
- Flow Cytometry
- Immunocytochemistry
应用说明 Flow Cytometry
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.生物信息 表位 Cytoplasmic 克隆 JSB-1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170 kDa P-gp, member of the superfamily of transmembrane transporters. JSB-1 detects P-gp overexpression in human tumor cells of all different histogenetic derivations.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.同种型 IgG1 品种反应性 HamsterHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000927.3
Entrez基因汇总 The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. 基因符号 - EC 3.6.3.44
- ABCB1
- PGY1
- MGC163296
- GP170
- ABC20
- CD243
- P-gp
- MDR1
- CLCS
纯化方法 Unpurified UniProt编号 - P08183
UniProt汇总 FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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