merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB3878,Anti-MyoD1 Antibody

  • merck millipore,默克密理博,MAB3878,Anti-MyoD1 Antibody

    产品名称：Anti-MyoD1 Antibody
    产品型号：MAB3878
    Anti-MyoD1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of MyoD1 & has been validated in ICC, IHC, IP & WB.

    merck millipore,默克密理博,MAB3878,Anti-MyoD1 Antibody

  • 产品介绍
  • merck millipore,默克密理博,MAB3878,Anti-MyoD1 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, Ch, H, MWB, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB3878
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-MyoD1 Antibody
    产品信息
    格式 Purified
    控制
    • Fetal Muscle, Rhabdomyosarcoma
    演示 Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
    应用
    应用 Anti-MyoD1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of MyoD1 & has been validated in ICC, IHC, IP & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Western blot: 1 μg/mL. Reacts with the ~45 kD protein.

    Immunohistochemistry on frozen and formalin-fixed, paraffin embedded tissue: 2-4 μg/mL.

    Immunocytochemistry

    Immunoprecipitation: 2 μg/mg of protein lysate

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Recombinant protein from mouse MyoD1.
    克隆 5.2F
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes MyoD1. The epitope is localized to amino acids 3-56 in the N-terminus of mouse MyoD1 protein.
    同种型 IgG2a
    品种反应性 RatChickenHumanMouse
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_002478.4
    Entrez基因汇总 This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
    基因符号
    • MYF3
    • MYOD1
    • MYOD
    • PUM
    • Myf-3
    纯化方法 Protein A purfied
    UniProt编号
    • P15172
    UniProt汇总 FUNCTION: SwissProt: P15172 # Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
    SIZE: 319 amino acids; 34490 Da
    SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1.
    SUBCELLULAR LOCATION: Nucleus.
    PTM: Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). & Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
    SIMILARITY: SwissProt: P15172 ## Contains 1 basic helix-loop-helix (bHLH) domain.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB3878,Anti-MyoD1 Antibody

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