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merck millipore,默克密理博,MAB3839,Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3
产品名称：Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3
产品型号：MAB3839
Detect Tissue Transglutaminase using this Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 validated for use in IC.
merck millipore,默克密理博,MAB3839,Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3839 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 Alternate Names - TGase C
- tTG
- Protein-glutamine gamma-Glutamyltransferase
- Transglutaminase 2
背景信息 Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues (Monsonego, 1997). Extracellular tTG is able to bind to and cross-link several ECM proteins and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation.
High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.产品信息 格式 Purified 演示 Protein A purified immunoglobulin in 0.02M Phosphate Buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide. 应用 应用 Detect Tissue Transglutaminase using this Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 validated for use in IC. 主要应用 - Immunocytochemistry
应用说明 Immunohistochemistry: 20 μg/mL
Adhesion Blocking: 10-20 μg/mL (per 2x10e5 cells). 4G3 interferes with adhesion of TPA stimulated THP-1 cells and M-CSF stimulated peripheral blood monocytes to fibronectin.
Optimal working dilutions must be determined by the end user.
PROTOCOL:
IMMUNOCYTOCHEMISTRY: THP-1 cells were induced to differentiate into macrophages by treatment with 150 ng/ml of TPA and plated on fibronectin coated glass coverslips for 72 hours.Live non-permeablized cells were incubated with 20 μg/mL of MAB3839 for 45 minutes, washed three times with PBS and fixed for 10 minutes with 3.7% paraformaldehyde. The cells were then washed with PBS and incubated 45 minutes with a fluoresceine labeled affinity-purified goat-anti-mouse secondary antibody. After extensive washing with PBS, cells were mounted in Fluorsave medium.
Observed Staining:Cell surface tTG was colocalized with b1- and b3-Integrins in podosome-like adhesive structures of macrophages adherent on fibronectin.生物信息 免疫原品种 Fibronectin binding domain of tissue transglutaminase (amino acids 1-165 of tTG). 表位 FN binding domain 克隆 4G3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Tissue Transglutaminase. Will recognize all three known splice variants of tTG. 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_198951.1
- NM_004613.2
Entrez基因汇总 Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - EC 2.3.2.13
- TGM2
- TG
- TGC
- TG2
- C
- protein-glutamine-gamma-glutamyltransferase
- Transglutaminase-2
- TGase-H
UniProt编号 - P21980
UniProt汇总 FUNCTION: SwissProt: P21980 # Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
SIZE: 687 amino acids; 77329 Da
SUBUNIT: Monomer.
SIMILARITY: SwissProt: P21980 ## Belongs to the transglutaminase superfamily. Transglutaminase family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,PF125,BACE1, His•Tag, Human, Recombinant, NSO Cells
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