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merck millipore,默克密理博,AB1991,Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat
产品名称：Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat
产品型号：AB1991
Detect Neurofilament H (200 kDa) using this Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat validated for use in IH(P), WB, IH.
merck millipore,默克密理博,AB1991,Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rp, Av, Ma IHC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1991 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat 背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section. 产品信息 格式 Serum 演示 Neat rabbit antisera. 应用 应用 Detect Neurofilament H (200 kDa) using this Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat validated for use in IH(P), WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry: 1:250-1:1,000 AB1991 is not affected by the level of neurofilament phosphorylation and is particularly good for revealing dendritic and perikaryal neurofilaments.
Immunoblotting
Electron microscopy
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 E. Coli recombinant fusion protein containing 37 lysine-serine-proline repeats of rat NF-H. 表位 lysine-serine-proline repeat 宿主 Rabbit 特异性 Strong reactivity to the major neurofilament subunit HF-H. Since a second neurofilament subunit, NF-M, also contains a few lysine-serine-proline (KSP) sequences, there is generally some reactivity with this protein also. AB1991 stains both phosphorylated and dephosphorylated neurofilaments. 品种反应性 ReptileAvianMammals 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_021076.2
基因符号 - KIAA0845
- NEFH
- NFH
- NF-H
UniProt编号 - P12036
UniProt汇总 FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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下一件merck millipore产品：merck millipore,默克密理博,12-372,Histone H4 Peptide, biotin conjugate, residues 2-24
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