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描述 概述 Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Supplied as a mixture of the ~68-70 kDa proenzyme and the ~65-67 kDa mature enzyme. BACE-1 is an aspartic protease that has been shown to cleave the amyloid precursor protein β site in the brain to generate the amyloid β peptide. MW: ~65-70 kDa under reducing conditions. 产品目录编号 PF125 品牌系列 Calbiochem®
同义词 Human β-Secretase 参考 参考 Ermolieff, J. et al. 2000. Biochemistry 39, 12450.
Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA 97, 1456.
Sinha, S. et al. 1999. Nature 402, 537.
Vassar, R. et al. 1999. Science 286, 735.
Yan, R. et al. 1999. Nature 402, 533.产品信息 活性 ≥1 pmol/min/µg as measured by the cleavage of 10 µM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH₂ fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature. 形式 Lyophilized 配方 Lyophilized from sterile-filtered PBS. 防腐剂 None 生物信息 纯度 ≥90% by SDS-PAGE 特异活性 ≥1 pmol/min/µg protein 理化信息 污染物 Endotoxin: ≤1 EU/µg BACE-1 溶解度 H₂O 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 4 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
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