• merck millipore,默克密理博,PF125,BACE1, His•Tag, Human, Recombinant, NSO Cells
  • merck millipore,默克密理博,PF125,BACE1, His•Tag, Human, Recombinant, NSO Cells

    产品名称:BACE1, His•Tag, Human, Recombinant, NSO Cells
    产品型号:PF125


    merck millipore,默克密理博,PF125,BACE1, His•Tag, Human, Recombinant, NSO Cells

  • 产品介绍
  • merck millipore,默克密理博,PF125,BACE1, His•Tag, Human, Recombinant, NSO Cells

    重要规格表

    描述
    概述 Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Supplied as a mixture of the ~68-70 kDa proenzyme and the ~65-67 kDa mature enzyme. BACE-1 is an aspartic protease that has been shown to cleave the amyloid precursor protein β site in the brain to generate the amyloid β peptide. MW: ~65-70 kDa under reducing conditions.
    产品目录编号 PF125
    品牌系列 Calbiochem®
    同义词 Human β-Secretase
    参考
    参考 Ermolieff, J. et al. 2000. Biochemistry 39, 12450.
    Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA 97, 1456.
    Sinha, S. et al. 1999. Nature 402, 537.
    Vassar, R. et al. 1999. Science 286, 735.
    Yan, R. et al. 1999. Nature 402, 533.
    产品信息
    活性 ≥1 pmol/min/µg as measured by the cleavage of 10 µM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH₂ fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature.
    形式 Lyophilized
    配方 Lyophilized from sterile-filtered PBS.
    防腐剂 None
    生物信息
    纯度 ≥90% by SDS-PAGE
    特异活性 ≥1 pmol/min/µg protein
    理化信息
    污染物 Endotoxin: ≤1 EU/µg BACE-1
    溶解度 H₂O
    储存和货运信息
    货运代码 Blue Ice Only
    毒性 Standard Handling
    储存 -20°C
    无需冷冻 No
    特殊指南 Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 4 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

    merck millipore,默克密理博,PF125,BACE1, His•Tag, Human, Recombinant, NSO Cells

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