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merck millipore,默克密理博,MAB367,Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
产品名称：Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
产品型号：MAB367
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.
merck millipore,默克密理博,MAB367,Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, Ma, Mk IHC, ICC R Purified Monoclonal Antibody 描述 产品目录编号 MAB367 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6. 应用 应用 Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP. 主要应用 - Immunohistochemistry
- Immunocytochemistry
不建议的应用 - Immunohistochemistry (Paraffin)
应用说明 Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells
Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).生物信息 免疫原品种 i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase. 克隆 M2-2-B3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.
SPECIES REACTIVITIES:
Expected to react with most mammalian species.同种型 IgG2a 品种反应性 RatHumanMammalsMonkey 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001006632.1
- NM_000739.2
- NM_001006626.1
- NM_001006627.1
- NM_001006628.1
- NM_001006629.1
- NM_001006630.1
- NM_001006631.1
Entrez基因汇总 The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. 基因符号 - HM2
- CHRM2
- MGC120006
- FLJ43243
- MGC120007
UniProt编号 - P08172
UniProt汇总 FUNCTION: SwissProt: P08172 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
SIZE: 466 amino acids; 51715 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P08172 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months. 包装信息 数量 100 µg
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