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merck millipore,默克密理博,MAB030,Anti-DNA Antibody, double stranded, clone BV16-13
产品名称：Anti-DNA Antibody, double stranded, clone BV16-13
产品型号：MAB030
Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
merck millipore,默克密理博,MAB030,Anti-DNA Antibody, double stranded, clone BV16-13
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A ICC, ELISA M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB030 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-DNA Antibody, double stranded, clone BV16-13 产品信息 格式 Culture Supernatant 演示 Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL. 应用 应用 Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC. 主要应用 - Immunocytochemistry
- ELISA
应用说明 EIA/RIA
Immunocytochemistry
Optimal working dilutions must be determined by end user.生物信息 表位 double stranded, 28% reactive with single stranded 克隆 BV16-13 宿主 Mouse 特异性 Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA同种型 IgG2aκ 品种反应性 All 抗体类型 Monoclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months. 包装信息 数量 500 µL
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