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品种反应性 主要应用 宿主 格式 抗体类型 R, B, H IHC, ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3300 Replaces 04-1133 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-TIMP-1 Antibody, clone 7-6C1 Alternate Names - Tissue Inhibitor of Metalloproteinase-1
背景信息 Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues is low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced. 产品信息 格式 Purified 演示 Purified immunoglobulin presented as a liquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing protease free bovine serum albumin. 应用 应用 Detect TIMP-1 using this Anti-TIMP-1 Antibody, clone 7-6C1 validated for use in ELISA, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Immunoblotting: 1:1000, identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues.Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein CC3328.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Bovine TIMP-1 克隆 7-6C1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 MAB3300 is a purified mouse monoclonal antibody to bovine tissue inhibitor of metalloproteinases-1 (bovine TIMP-1). The antibody specifically reacts with bovine TIMP-1 and cross-reacts with human and rat TIMP-1, but not with mouse TIMP-1. By western blot the antibody recognizes a glycoprotein of 28.5 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1). 同种型 IgG1κ 品种反应性 RatBovineHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003254.2
Entrez基因汇总 This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. 基因符号 - HCI
- TIMP1
- TIMP
- FLJ90373
- CLGI
- EPA
- EPO
- TIMP-1
非反应性品种 - Mouse
UniProt编号 - P01033
UniProt汇总 FUNCTION: SwissProt: P01033 # Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
SIZE: 207 amino acids; 23171 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.
SIMILARITY: SwissProt: P01033 ## Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.产品使用声明 销售限制 This product is not available for sale in Japan. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. 包装信息 数量 100 µg
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