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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1871 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Caspase 1 Antibody Alternate Names - ICE
背景信息 Caspase-1 (ICE, interleukin-1b-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin-1b and IL-18 into active cytokines. Like other caspases, caspase-1 itself is synthesized as a proenzyme that is cleaved during activation into large (20 kDa) and small (10 kDa) subunits. 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS, pH 7.2, containing 50% glycerol, 1% BSA and 0.02% sodium azide. 应用 应用 This Anti-Caspase 1 Antibody is validated for use in IF, IP, WB for the detection of Caspase 1. 主要应用 - Western Blotting
应用说明 Western blot: 1-4 μg/mL
Immunoprecipitation: 4-8 μg/mL
Immunofluorescence: 10-20 μg/mL
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB1871 recognizes the 45 kDa procaspase-1 as well as the 20 kDa cleaved caspase-1. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_033295.2
- NM_001223.3
- NM_033292.2
- NM_033293.2
- NM_033294.2
Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. 基因符号 - Caspase-1 subunit p10].
- CASP1
- IL1BC
- p45
- ICE
- IL1BCE
- P45
- IL-1BC
- IL1B-convertase
- CASP-1
- EC 3.4.22.36 [Contains: Caspase-1 subunit p20
UniProt编号 - P29466
UniProt汇总 FUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
SIZE: 404 amino acids; 45159 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C. For optimal storage, aliquot and freeze at -20°C to -70°C. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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