merck millipore,默克密理博,HTS031M,ChemiSCREEN™ CRTH2 Membrane Preparation

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merck millipore,默克密理博,HTS031M,ChemiSCREEN™ CRTH2 Membrane Preparation
产品名称：ChemiSCREEN™ CRTH2 Membrane Preparation
产品型号：HTS031M
Human CRTH2 / DP2 / GPR44 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.

merck millipore,默克密理博,HTS031M,ChemiSCREEN™ CRTH2 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS031M,ChemiSCREEN™ CRTH2 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPgammaS binding.	Human	Chem-1	CRTH2 / DP2 / GPR44

描述
产品目录编号	HTS031M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ CRTH2 Membrane Preparation
概述	Human CRTH2
背景信息	Chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) is a GPCR related to FPR-like chemoattractant receptors that is highly expressed on Th2 cells, eosinophils, basophils, and type 2 cytotoxic T cells (Nagata et al., 1999). The most potent ligand for CRTH2 is the prostaglandin PGD₂, a major mast cell-derived allergic mediator (Hirai et al., 2001). Another GPCR, DP, is activated by PGD₂, and the relative contributions of DP and CRTH2 to PGD₂-mediated allergic disease are being elucidated. A small molecule antagonist of CRTH2, ramatroban, attenuates PGD₂-mediated bronchial hyperresponsiveness and antigen-induced inflammation (Sugimoto et al., 2003; Shichijo et al., 2003).Chemicon's CRTH2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PGD₂/CRTH2 interactions. The membrane preparations exhibit a Kd of 10 nM for [³H]-Prostaglandin D₂. With 2.5 μg/well CRTH2 Membrane Prep and 10 nM [³H]-Prostaglandin D₂, a greater than 5-fold signal-to-background ratio is obtained.

产品信息
格式	Membranes
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human CRTH2 / DP2 / GPR44 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
应用说明	Radioligand binding assay, and GTPgammaS binding.

生物信息
品种	Human
品种反应性	Human
Entrez基因编号	NM_004778.2
Entrez基因汇总	G protein-coupled receptors (GPCRs), such as GPR44, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.[supplied by OMIM]
基因符号	CD294 GPR44 DL1R CRTH2
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	CRTH2 / DP2 / GPR44
靶子系列	Prostanoid
UniProt编号	Q9Y5Y4
UniProt汇总	FUNCTION: SwissProt: Q9Y5Y4 # Orphan receptor. SIZE: 395 amino acids; 43240 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: Q9Y5Y4 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50mM HEPES, 10mM MnCl2, 1mM EDTA, 0.2% BSA, pH to 7.0 and filter. Wash buffer: 50mM HEPES, 0.5M NaCl, pH to 7.4 and filter. Radioligand: [3H] Prostaglandin D2 (Amersham#: TRK734)

产品使用声明

质量保证

Table 1. Signal:background and specific binding values obtained in a competition binding assay with CRTH2 membrane preparation with unlabeled Prostaglandin D₂.

	10 µg/well
Signal:Background	12.2
Specific Binding (cpm)	4307

Table 2. IC50 values for ligands obtained in a competition binding assay with CRTH2 membrane preparation.

	IC50 (nM)
Prostaglanding D₂	4.5
13,14-dihydro-15-keto prostaglandin D₂	14.7
BW A868C	7429

SPECIFICATIONS: 1 unit = 10 µg
Bmax: 13.93 pmol/mg
Kd: 10 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.