merck millipore,默克密理博,GF002,Fibroblast Growth Factor acidic Protein, Recombinant human

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,GF002,Fibroblast Growth Factor acidic Protein, Recombinant human
产品名称：Fibroblast Growth Factor acidic Protein, Recombinant human
产品型号：GF002
The Fibroblast Growth Factor-acidic (or FGF-a protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothe More>>

merck millipore,默克密理博,GF002,Fibroblast Growth Factor acidic Protein, Recombinant human

产品介绍

merck millipore,默克密理博,GF002,Fibroblast Growth Factor acidic Protein, Recombinant human

重要规格表

主要应用	品种	来源	纯度
CULT	Human	E.coli	Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg of FGF-a. Sterile filtered.

描述
产品目录编号	GF002
品牌系列	Chemicon®
商名	Chemicon
描述	Fibroblast Growth Factor acidic Protein, Recombinant human
概述	Fibroblast Growth Factor-acidic (FGF-a) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Human FGF-acidic is a 15.8 kDa protein containing 140 amino acid residues.
Alternate Names	alpha FGF FGF-1

产品信息
演示	Sterile filtered. Lyophilized from 5mM Sodium Phosphate, pH 7.2 + 100mM NaCl.

应用
应用	The Fibroblast Growth Factor-acidic (or FGF-a protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
主要应用	Cell Culture
应用说明	For most in vitro applications, FGF-a exerts its biological activity in the concentration range of 1.0 to 50 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Optimal working dilutions must be determined by end user.

生物信息
纯度	Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg of FGF-a. Sterile filtered.
来源	E.coli
品种	Human
特异活性	The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 10.0 ng/mL, corresponding to a specific activity of > 1 x 10e5 units/mg.
Entrez基因编号	NM_033137.1 NM_000800.2 NM_033136.1
Entrez基因汇总	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described.
基因符号	AFGF FGF1 ECGFA HBGF1 FGFA ECGF-beta FGF-alpha aFGF GLIO703 HBGF-1 ECGF ECGFB
UniProt编号	P05230
UniProt汇总	FUNCTION: SwissProt: P05230 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SIZE: 155 amino acids; 17460 Da SUBUNIT: Monomer. Binds FGFR2. Forms a ternary complex containing 2 molecules each of FGFR2 and FGF1 for 1 heparin molecule. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. SIMILARITY: SwissProt: P05230 ## Belongs to the heparin-binding growth factors family. MISCELLANEOUS: This protein binds heparin, although less strongly than does bFGF.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The lyophilized FGF-a, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstitute in 5mM Sodium Phosphate, pH 7.0-7.2 to a concentration of >0.5 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted FGF-a should be stored in working aliquots at -20°C. Repeated freeze/thaw cycles will result in some loss of activity.

储存和货运信息

存储条件

The lyophilized FGF-a, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstitute in 5mM Sodium Phosphate, pH 7.0-7.2 to a concentration of >0.5 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted FGF-a should be stored in working aliquots at -20°C. Repeated freeze/thaw cycles will result in some loss of activity.