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merck millipore,默克密理博,MABS191,Anti-phospho-VEGFR2 (Tyr1212) Antibody, clone 12A10.1
产品名称：Anti-phospho-VEGFR2 (Tyr1212) Antibody, clone 12A10.1
产品型号：MABS191
This Anti-phospho-VEGFR2 (Tyr1212) Antibody, clone 12A10.1 is validated for use in WB for the detection of phospho-VEGFR2 (Tyr1212).
merck millipore,默克密理博,MABS191,Anti-phospho-VEGFR2 (Tyr1212) Antibody, clone 12A10.1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, Po WB M Purified Monoclonal Antibody 描述 产品目录编号 MABS191 描述 Anti-phospho-VEGFR2 (Tyr1212) Antibody, clone 12A10.1 Alternate Names - CD309
- Vascular endothelial growth factor receptor 2
- VEGFR-2
- Fetal liver kinase 1
- FLK-1
- Kinase insert domain receptor
- KDR
- Protein-tyrosine kinase receptor flk-1
背景信息 KDR/Flk-1/VEGFR-2 is a putative receptor protein tyrosine kinase expressed in endothelial cells of the developing embryo. Vascular endothelial growth factor (VEGF) and VEGFR2 have been implicated as the key endothelial cell-specific factor signaling pathway required for pathological angiogenesis, including tumor neovascularization. Inhibition of the VEGF tyrosine kinase signaling pathway blocks new blood vessel formation in growing tumors, leading to stasis or regression of tumor growth. 产品信息 格式 Purified 控制 - Untreated and Colony-stimulating factor (CSF) treated Porcine Aortic Endothelial (PAE) cell lysates expressing VEGFR2.
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 This Anti-phospho-VEGFR2 (Tyr1212) Antibody, clone 12A10.1 is validated for use in WB for the detection of phospho-VEGFR2 (Tyr1212). 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human VEGFR2 phosphorylated at Tyr1212. 表位 Phosphorylated Tyr1212 克隆 12A10.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes VEGFR2 phosphorylated at Tyr1212. 同种型 IgMκ 品种反应性 HumanPig Species Reactivity Note Demonstrated to react with Porcine. Predicted to react with Human based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002244
Entrez基因汇总 Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. 基因符号 - VEGFR2
- KDR
- FLK1
修改 - Phosphorylation
纯化方法 Purified ion exchange chromatography UniProt编号 - P35968
UniProt汇总 FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic haematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK and SRC.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
ENZYME REGULATION: Present in an inactive conformation in the absence of bound ligand. Binding of VEGFA, VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by the small molecule PTK inhibitor SU5614 ((3Z)-5-Chloro-3-[(3,5-dimethyl-1H-pyrrol-2-yl)methylene]-1,3-dihydro-2H-indol-2-one).
SUBUNIT STRUCTURE: Interacts with MYOF. Interacts with VEGFA, VEGFC and VEGFD. Monomer in the absence of bound VEGFA, VEGFC or VEGFD. Homodimer in the presence of bound dimeric VEGFA, VEGFC or VEGFD. Can also form heterodimers with FLT1 and FLT4. Interacts (tyrosine phosphorylated) with FYN, NCK1, PLCG1 and SHB. Interacts with HIV-1 Tat. Interacts with CBL. Interacts with SH2D2A/TSAD and GRB2.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Note: Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus. Isoforms 2 and 3 are secreted.
TISSUE SPECIFICITY: Detected in cornea (at protein level). Widely expressed.产品使用声明 质量保证 Evaluated by Western Blot in untreated and Colony-stimulating factor (CSF) treated Porcine Aortic Endothelial (PAE) cell lysates expressing VEGFR2.
Western Blot Analysis: 0.05 µg/mL of this antibody detected VEGFR2 in 10 µg of untreated and Colony-stimulating factor (CSF) treated Porcine Aortic Endothelial (PAE) cell lysates expressing VEGFR2.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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