merck millipore,默克密理愽
  • merck millipore,默克密理博,17-329,HAT Assay Reagent Kit

  • merck millipore,默克密理博,17-329,HAT Assay Reagent Kit

    产品名称：HAT Assay Reagent Kit
    产品型号：17-329
    Accessory reagent kit for the analysis of histone acetyltransferase activity.

    merck millipore,默克密理博,17-329,HAT Assay Reagent Kit

  • 产品介绍
  • merck millipore,默克密理博,17-329,HAT Assay Reagent Kit

    重要规格表

    主要应用
    HAT
    描述
    产品目录编号 17-329
    品牌系列 Upstate
    商名
    • Upstate
    描述 HAT Assay Reagent Kit
    概述 Accessory reagent kit for the analysis of histone acetyltransferase activity.
    产品信息
    部件
    • Acetyl-CoA
    • HAT Assay Buffer, 5X
    • Core Histones
    • P81 Phosphocellulose squares
    应用
    应用 Accessory reagent kit for the analysis of histone acetyltransferase activity.
    主要应用
    • Histone Acetyltransferase Assay (HAT)
    应用说明 Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). Use 1- 20μl per assay point in the HAT Reaction Cocktail below. Optimal nuclear extract concentration will need to be determined empirically.
    生物信息
    Entrez基因编号
    • NM_003642.2
    • NM_001033085.1
    Entrez基因汇总 Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
    基因符号
    • HAT1
    UniProt编号
    • O14929
    UniProt汇总 FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at 'Lys-5' and 'Lys-12' and acetylates histone H2A at 'Lys-5'. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
    SIZE: 419 amino acids; 49513 Da
    SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
    SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
    SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
    产品使用声明
    质量保证 Routinely evaluated by incubating pCAF, active (14-309) with Core Histones (13-107B). Reactions were terminated at indicated times and acetyl incorporation was measured.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 kit
    包装 Kit capacity: 100 assays

    merck millipore,默克密理博,17-329,HAT Assay Reagent Kit

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