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品种反应性 主要应用 宿主 格式 抗体类型 Sh, B, Ca, H, M, Po, R, Rb WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB326 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CNPase Antibody, clone 11-5B Alternate Names - 2'3'-Cyclic Nucleotide 3'-Phosphohydrolase
背景信息 CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.产品信息 格式 Purified 控制 - Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
演示 Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide 应用 应用 Detect CNPase using this Anti-CNPase Antibody, clone 11-5B validated for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry:
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified human brain CNPase 克隆 11-5B 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc. 同种型 IgG1 品种反应性 SheepBovineCanineHumanMousePigRatRabbit Species Reactivity Note Bovine, canine, human, mouse, porcine, rat, rabbit and sheep. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_033133.4
基因符号 - EC 3.1.4.37
- CNP
- CNPase
- CNP1
非反应性品种 - Guinea Pig
- Chicken
纯化方法 Protein A purfied UniProt编号 - P09543
UniProt汇总 SIZE: 421 amino acids; 47579 Da
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.产品使用声明 质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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