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merck millipore,默克密理博,MAB3034,Anti-DNA Antibody, single stranded, clone 16-19
产品名称:Anti-DNA Antibody, single stranded, clone 16-19
产品型号:MAB3034
Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.
merck millipore,默克密理博,MAB3034,Anti-DNA Antibody, single stranded, clone 16-19
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB3034 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-DNA Antibody, single stranded, clone 16-19 产品信息 格式 Purified 演示 0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide. 应用 应用 Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA. 主要应用 - ELISA
应用说明 ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.生物信息 表位 single stranded DNA 克隆 16-19 宿主 Mouse 特异性 Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.
ANTIGEN BINDING PROFILE:
Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53
a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
BINDING ASSAY
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ISOELECTRIC POINT = 8.3同种型 IgG2aκ 品种反应性 All 抗体类型 Monoclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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