merck millipore,默克密理博,HTS210RTA,Ready-to-Assay™ P2Y2 Purinergic Receptor Frozen Cells

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	1321N1	P2Y2

描述
产品目录编号	HTS210RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ P2Y2 Purinergic Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The P2Y GPCRs serve as receptors for extracellular nucleotides (Abbracchio et al., 2006). P2Y2 (also known as P2U) is activated by UTP and ATP, and couples to Gq to increase intracellular calcium (Parr et al., 1994). The P2Y2 receptor knockout mouse displays defective nucleotide-stimulated Cl- secretion, and a P2Y2 selective agonist increases tracheal epithelial chloride and water secretion (Cressman et al., 1999; Yerxa et al., 2002). As a result, P2Y2 is a potential target for treatment of cystic fibrosis. In addition, the P2Y2 KO mouse displays salt-resistant hypertension (Rieg et al., 2007). Millipore’s cloned human P2Y2-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y2 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y2.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human P2Y2 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.