merck millipore,默克密理愽
  • merck millipore,默克密理博,MABE285,Anti-Replication Protein A Antibody, clone RPA34-20

  • merck millipore,默克密理博,MABE285,Anti-Replication Protein A Antibody, clone RPA34-20

    产品名称：Anti-Replication Protein A Antibody, clone RPA34-20
    产品型号：MABE285
    Use Anti-Replication Protein A Antibody, clone RPA34-20 (mouse monoclonal antibody) validated in WB, ICC, IHC to detect Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p More>>

    merck millipore,默克密理博,MABE285,Anti-Replication Protein A Antibody, clone RPA34-20

  • 产品介绍
  • merck millipore,默克密理博,MABE285,Anti-Replication Protein A Antibody, clone RPA34-20

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HIHC, WB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABE285
    描述 Anti-Replication Protein A Antibody, clone RPA34-20
    Alternate Names
    • RP-A p34
    • Replication protein A 32 kDa subunit
    • RP-A p32
    • Replication factor A protein 2
    • RF-A protein 2
    • Replication protein A 34 kDa subunit
    背景信息 RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.
    产品信息
    格式 Purified
    控制
    • HeLa cell lysate.B10
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Use Anti-Replication Protein A Antibody, clone RPA34-20 (mouse monoclonal antibody) validated in WB, ICC, IHC to detect Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Replication Protein A in HeLa and A431 cells.

    Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human colorectal adenocarcinoma tissues.
    生物信息
    免疫原品种 Replication Protein A purified from U293 cells
    克隆 RPA34-20
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with Human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_002937
    基因符号
    • RPA34
    • RPA2
    • REPA2
    • RPA32
    纯化方法 Protein G purified
    UniProt编号
    • P15927
    UniProt汇总 FUNCTION: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

    SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3.

    SUBCELLULAR LOCATION: Nucleus. Nucleus > PML body. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.

    POST-TRANSLATIONAL MODIFICATION: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as an hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa cell lysate.

    Western Blot Analysis: 1 µg/mL of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MABE285,Anti-Replication Protein A Antibody, clone RPA34-20

上一件merck millipore产品：merck millipore,默克密理博,557330,Rolipram - CAS 61413-54-5 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,218776,Caspase-9 Inhibitor II, Cell-Permeable - Calbiochem


相关产品

merck millipore,默克密理博,MR-015P-5D,EmbryoMax M2 Medium (1X), Powdered, with phenol red, 250ml merck millipore,默克密理博,MR-015P-5D,EmbryoMax M2 Medium (1X), Powdered, with phenol red, 250ml

merck millipore,默克密理博,MAB3034,Anti-DNA Antibody, single stranded, clone 16-19 merck millipore,默克密理博,MAB3034,Anti-DNA Antibody, single stranded, clone 16-19

merck millipore,默克密理博,GF436,HumanKine PDGFaa, Human Recombinant Xeno-Free merck millipore,默克密理博,GF436,HumanKine PDGFaa, Human Recombinant Xeno-Free

merck millipore,默克密理博,AX90010,AXIS™ Axon Isolation Device, 900 µm merck millipore,默克密理博,AX90010,AXIS™ Axon Isolation Device, 900 µm

merck millipore,默克密理博,557330,Rolipram - CAS 61413-54-5 - Calbiochem merck millipore,默克密理博,557330,Rolipram - CAS 61413-54-5 - Calbiochem

merck millipore,默克密理博,218776,Caspase-9 Inhibitor II, Cell-Permeable - Calbiochem merck millipore,默克密理博,218776,Caspase-9 Inhibitor II, Cell-Permeable - Calbiochem

merck millipore,默克密理博,05-443,Anti-VEGF Antibody, clone JH merck millipore,默克密理博,05-443,Anti-VEGF Antibody, clone JH

merck millipore,默克密理博,AB3478,Anti-Defensin β3 Antibody merck millipore,默克密理博,AB3478,Anti-Defensin β3 Antibody

merck millipore,默克密理博,70980,Trail Mix™ Protein Markers merck millipore,默克密理博,70980,Trail Mix™ Protein Markers

merck millipore,默克密理博,AB9452,Anti-Muscarinic Acetylcholine Receptor M2 Antibody merck millipore,默克密理博,AB9452,Anti-Muscarinic Acetylcholine Receptor M2 Antibody


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部