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品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB2618 描述 Anti-Migfilin Antibody, clone 43.9 Alternate Names - mitogen-inducible 2 interacting protein
- CSX-associated LIM
- MIG2-interacting protein
- Mitogen-inducible 2-interacting protein
- filamin binding LIM protein 1
- filamin-binding LIM protein-1
背景信息 Migfilin is a recently identified protein involved in linking the plasma membrane to the actin cytoskeleton as a component of cell-cell and cell-ECM adhesions through the binding of filamin proteins and plays a role in the restructuring of the actin cytoskeleton and regulation of cell shape. A widely expressed protein, migfilin is composed of a filamin binding domain found in the N-terminus, a central proline-rich domain, and three LIM domains within the C-terminus. These LIM domains assist in kindlin-2 binding and are necessary components for localization to focal adhesions. Recent studies show that the central proline-rich domain binds to vasodilator-stimulated phosphoproteins, which seem to be a requirement of migfilin’s ability to affect cell migration. The N-terminal domain’s role in binding filamin is what allows migfilin to link with the actin cytoskeleton. Removal of the final LIM domain and the central proline-rich domain results in alternative splicing, and three isoforms of migfilin have been observed. All isoforms are shown to be expressed in kidney, pancreas, lung, placenta, heart and platelets. Isoform 2 has also been observed in brain and skeletal muscle tissue. Levels of migfilin considered to be higher than the normal levels of expression have been associated with human leiomyosarcoma. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This Anti-Migfilin Antibody, clone 43.9 is validated for use in WB for the detection of Migfilin. 主要应用 - Western Blotting
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human Migfilin. 表位 Unknown 克隆 43.9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody recognizes Migfilin. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_060026.2
Entrez基因汇总 This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. 基因符号 - Migfilin
- FBLIM1
- CAL
- FBLP-1
- FBLP1
纯化方法 Protein G purfied UniProt编号 - Q8WUP2
UniProt汇总 FUNCTION: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly.
SUBUNIT STRUCTURE: Isoform 1 and isoform 3 interact with PLEKHC1, FLNA and FLNC. Isoform 2 interacts with FLNB. Interacts with NKX2-5 by similarity.
SUBCELLULAR LOCATION: Cytoplasm › cell cortex. Cell junction › focal adhesion. Cytoplasm › cytoskeleton. Note: Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers.
TISSUE SPECIFICTY: Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.
SEQUENCE SIMILARITIES: Contains 3 LIM zinc-binding domains.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Migfilin in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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