merck millipore,默克密理博,04-1063,Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, rabbit monoclonal

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merck millipore,默克密理博,04-1063,Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, rabbit monoclonal
产品名称：Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, rabbit monoclonal
产品型号：04-1063
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-Met (Tyr1349) using this Anti-phospho More>>

merck millipore,默克密理博,04-1063,Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1063,Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1063
描述	Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, rabbit monoclonal
Alternate Names	oncogene MET HGF receptor HGF/SF receptor Met proto-oncogene tyrosine kinase SF receptor Scatter factor receptor met proto-oncogene met proto-oncogene (hepatocyte growth factor receptor)
背景信息	Met is a receptor protein kinase (RPTK) for a multifunctional cytokine called (HFG) which controls cell growth and motility. Met has unique features that distinguish it from other members of RPTK's.

产品信息
格式	Unpurified
控制	A431 cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-Met (Tyr1349) using this Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, Rabbit validated for use in WB, IH(P), IC & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Met in human breast carcinoma tissue. Immunocytochemistry Analysis: A 1:50 -100 dilution from a previous lot worked in IC. Immunoprecipitation: A 1:20 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Phospho-specific peptide corresponding to residues surrounding Tyr1349 of human Met.
表位	Phosphorylated Tyr1349
克隆	EP2367Y
宿主	Rabbit
特异性	This antibody detects Met phosphorylated at Tyr1349.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_000236.2
Entrez基因汇总	The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
基因符号	c-Met AUTS9 EC 2.7.10.1 HGFR OTTHUMP00000069168 RCCP2
修改	Phosphorylation
纯化方法	Unpurified
UniProt编号	P08581
UniProt汇总	FUNCTION: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 By similarity. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: The kinase domain is involved in SPSB1 binding. INVOLVEMENT IN DISEASE: Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. Defects in MET may be associated with gastric cancer. Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM:611015]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 3 IPT/TIG domains. Contains 1 protein kinase domain. Contains 1 Sema domain.

产品使用声明
质量保证	Evaluated by Western Blot on untreated and alkaline phosphatase-treated A431 cell lysates. Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect Met in A431 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.