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品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, FC, IHC R Purified Monoclonal Antibody 描述 产品目录编号 MAB1997 Replaces 04-1109 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin β1 Antibody, clone MB1.2 Alternate Names - integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
- CD29 antigen
- Fibronectin receptor subunit beta
- Integrin VLA-4 subunit beta
- fibronectin receptor beta subunit
- integrin VLA-4 beta subunit
- integrin beta 1
- integrin
- beta 1 (fibronectin receptor
- beta polypeptide
- antigen CD29 includes MDF2
背景信息 Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. 产品信息 格式 Purified 控制 - C2C12 lysate.
- Human OVCAR5 cell line whole cell lysate
演示 Purified rat monoclonal IgG2κ liquid in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl with 0.1% NaN3 应用 应用 Anti-Integrin β1 Antibody, clone MB1.2 is an antibody against Integrin β1 for use in FC, IH, IP & WB. 主要应用 - Western Blotting
- Flow Cytometry
- Immunohistochemistry
应用说明 Immunoprecipitation:
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.生物信息 克隆 MB1.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 Reacts with β1 subunit of VLA (β1) integrins 同种型 IgG2κ 品种反应性 RatMouse Species Reactivity Note Mouse. Expected to react with rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002202
Entrez基因汇总 Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. 基因符号 - VLA-BETA
- ITGB1
- GPIIA
- OTTHUMP00000063731
- OTTHUMP00000046253
- OTTHUMP00000063733
- VLAB
- OTTHUMP00000063732
- MDF2
- FNRB
- CD29
- MSK12
纯化方法 Protein A purfied UniProt编号 - P05556
UniProt汇总 FUNCTION: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation.
SUBUNIT STUCTURE: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5. Interacts with FLNA, FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with KRT1 in the presence of GNB2L1 and SRC. Interacts with HIV-1 Tat. Binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note= Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells.
TISSUE SPECIFICTY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).
PTM: The cysteine residues are involved in intrachain disulfide bonds By similarity.
SIMILARITY: Belongs to the integrin beta chain family.
Contains 1 VWFA domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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