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品种反应性 主要应用 宿主 格式 抗体类型 H, M ICC M Purified Monoclonal Antibody 描述 产品目录编号 04-966 描述 Anti-Cby1 Antibody, clone 8-2 Alternate Names - coiled-coil protein PIGEA-14 2 <br />polycystin-2 interactor, Golgi- and endoplasmic reticulum-associated protein, 14 kDa
- ARPP-binding protein
- Cytosolic leucine-rich protein
- PKD2 interactor, Golgi and endoplasmic reticulum-associated 1
- PKD2 interactor, golgi and endoplasmic reticulum associated 1
- chibby homolog 1 (Drosophila)
- chromosome 22 open reading frame 2
背景信息 Chibby (Cby) is a β-catenin antagonist that is evolutionarily conserved from fly to human. Cby interacts with β-catenin in a manner that competes with TCF/LEF, and thus represses β-catenin-mediated gene activation. Cby and 14-3-3 protein form a trimolecular complex with β-catenin. Cby and 14-3-3 collaborate to promote cytoplasmic localization of β-catenin and thereby inhibit β-catenin signalling. Cby acts, in concert with 14-3-3 proteins, to facilitate nuclear export of β-catenin and thereby antagonize β-catenin signalling. Therefore, the inhibition of β-catenin signalling by Cby involves at least two distinct molecular mechanisms: competing with TCF/LEF transcription factors for β-catenin in the nucleus and facilitating nuclear export of β-catenin through interaction with 14-3-3 following its phosphorylation by AKT/PKB. 产品信息 格式 Purified 控制 - NIH/3T3, A431, HeLa, and C2C12 cells
演示 Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This Anti-Cby1 Antibody, clone 8-2 is validated for use in IC, IP for the detection of Cby1. 主要应用 - Immunocytochemistry
应用说明 Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cby1 in NIH/3T3, A431, HeLa, and C2C12 cells.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Feng-Qian, L., et al. (2008). J. Cell Biol. 181(7):1141–1154.)
Western Blot Analysis: Western Blot is difficult to detect on endogenous lysates but has been shown to work in over expressed cell lines.生物信息 免疫原品种 Recombinant protein corresponding to human Cby1. 表位 Unknown 克隆 8-2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Cby1. 同种型 IgG2aκ 品种反应性 HumanMouse Species Reactivity Note Proven to react with human and mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_056188.1
Entrez基因汇总 Beta-catenin is a transcriptional activator and oncoprotein involved in the development of several cancers. The protein encoded by this gene interacts directly with the C-terminal region of beta-catenin, inhibiting oncogenic beta-catenin-mediated transcriptional activation by competing with transcription factors
for binding to beta-catenin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].基因符号 - arb1
- CBY1
- ARB1
- CBY
- Cby
- Chibby
- PGEA1
- PIGEA-14
- PIGEA14
纯化方法 Protein G purfied UniProt编号 - Q9Y3M2
UniProt汇总 FUNCTION: Inhibits the Wnt/Wingless pathway by binding to beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
SUBUNIT STRUCTURE: Homodimer; interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of beta-catenin.
SUBCELLULAR LOCATION: Nucleus speckle. Golgi apparatus › trans-Golgi network. Note: Nuclear, in a punctate manner. Also found in the trans-Golgi.
TISSUE SPECIFICITY: Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.
MISCELLANEOUS: Chibby is Japanese for 'small'; the gene was so named for the RNAi phenotype seen in flies.
SEQUENCE SIMILARITIES: Belongs to the chibby family.产品使用声明 质量保证 Evaluated by Immunocytochemistry Analysis on NIH/3T3 cells.
Immunocytochemistry Analysis: This antibody positively stains the cytoplasm. The nucleus is also stained with less intensity.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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