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merck millipore,默克密理博,MAB13444,Anti-Integrin α6 Antibody, clone BnC6B4
产品名称：Anti-Integrin α6 Antibody, clone BnC6B4
产品型号：MAB13444
Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH.
merck millipore,默克密理博,MAB13444,Anti-Integrin α6 Antibody, clone BnC6B4
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ch IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB13444 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin α6 Antibody, clone BnC6B4 Alternate Names - CD49f
产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl,pH=7.6, 0.1% Sodium azide. 应用 应用 Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Western blot
Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.
Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.
Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).
Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit. 克隆 BnC6B4 宿主 Mouse 特异性 Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots. 同种型 IgG1 品种反应性 Chicken 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001079818.1
- NM_000210.2
Entrez基因汇总 The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - CD49f
- ITGA6
- VLA-6
- ITGA6B
UniProt编号 - P23229
UniProt汇总 FUNCTION: SwissProt: P23229 # Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.
SIZE: 1130 amino acids; 126619 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
PTM: Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
DISEASE: "SwissProt: P23229 # Defects in ITGA6 are a cause of epidermolysis bullosa with pyloric atresia (EB-PA) [MIM:226730]; also known as aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive disease characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus."
SIMILARITY: SwissProt: P23229 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Manufactured by Daiichi Fine Chemical Co., Ltd
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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