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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-350 Replaces 04-323 品牌系列 Upstate
商名 - Upstate
描述 Anti-AMPK α1 Antibody Alternate Names - protein kinase, AMP-activated, alpha 1 catalytic subunit
- 5'-AMP-activated protein kinase, catalytic alpha-1 chain
- AMP -activate kinase alpha 1 subunit
- AMP-activated protein kinase, catalytic, alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
- alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
- protein kinase
- AMP-activated
背景信息 AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity. 产品信息 格式 Purified 控制 - A431cell lysate, or lysate of HeLa cells treated with heat shock
Included Positive Antigen Control: Catalog # 12-301, Non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Protein A purified rabbit in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. 应用 应用 This Anti-AMPK α1 Antibody is validated for use in WB for the detection of AMPK α1. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to amino acids 376-392 (C-ARHTLDELNPQKSKHQG-COOH) of human AMP-activated Protein Kinase α1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AMPKα1 同种型 IgG 品种反应性 MouseHumanRat Species Reactivity Note Human, rat, and mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006242
Entrez基因汇总 The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - AMPK1
- PRKAA1
- MGC57364
- AMPK
- MGC33776
- AMPKa1
纯化方法 Protein A chromatography UniProt编号 - Q13131
UniProt汇总 FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
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