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品种反应性 主要应用 宿主 格式 抗体类型 H FC, IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB1164 Replaces 04-214 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-c-Kit Antibody, clone 1DC3 Alternate Names - SCF Receptor
- CD117
背景信息 c-Kit is a 145 kDa glycosylated transmembrane protein whose extracellular domain contains Ig-like domains that bind c-Kit ligand (KL; stem cell factor) to initiate signaling that programs mast cell proliferation, differentiation, migration, and survival. 产品信息 格式 Purified 控制 - CCRF-HSB-2 (human leukemic lymphoblast) whole cell lysates
演示 Purified Immunoglobulin. Liquid in 0.02M phosphate buffer with 0.25 M NaCl, pH=7.6 with 0.1% sodium Azide added as a preservative. 应用 应用 This Anti-c-Kit Antibody, clone 1DC3 is validated for use in FC, IH, IP, WB for the detection of c-Kit. 主要应用 - Flow Cytometry
- Immunohistochemistry
- Western Blotting
应用说明 Flow Cytometry: 1 μg/test (100 μL) (Caruana, 1999)
Immunohistochemistry on cell smears (Ferrao, 1997)
Western blotting (non-reducing conditions)
Immunoprecipitation
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human erythroleukemia cell line. 克隆 1DC3 宿主 Mouse 特异性 Recognizes c-Kit (CD117, Steel factor receptor, and stem cell factor receptor), a transmembrane receptor tyrosine kinase expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and its ligand is required for normal hematopoiesis, mast cell differentiation, melanogenesis, and gametogenesis. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Does not block the function of the c-Kit receptor (Aylett, 1995). 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001093772.1
- NM_000222.2
Entrez基因汇总 This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. 基因符号 - EC 2.7.10.1
- KIT
- C-Kit
- PBT
- CD117
- SCFR
- c-kit
UniProt编号 - P10721
UniProt汇总 FUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
SIZE: 976 amino acids; 109865 Da
SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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