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merck millipore,默克密理博,HTS223M,ChemiSCREEN™ Membrane Preparation Recombinant Human D4 Dopamine Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human D4 Dopamine Receptor
产品型号：HTS223M
Human D4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS223M,ChemiSCREEN™ Membrane Preparation Recombinant Human D4 Dopamine Receptor
- 产品介绍
- merck millipore,默克密理博,HTS223M,ChemiSCREEN™ Membrane Preparation Recombinant Human D4 Dopamine Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 D4 描述 产品目录编号 HTS223M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human D4 Dopamine Receptor 概述 Full-length human DRD4.4 cDNA encoding the D4 isoform with 4 andem repeats in the third cytoplasmic loop 背景信息 The neurotransmitter dopamine is involved in a wide variety of neuroendocrine, emotional, cognitive and locomotor functions. These activities of dopamine are mediated by a group of 5 G protein coupled receptors, 2 of which (D1 and D5) couple to Gs and 3 of which (D2, D3, D4) couple to Gi (Missale et al., 1998). The D4 receptor is highly polymorphic, particularly in the third cytoplasmic loop, which contains from 2 to 7 repeats of 16 amino acids. Individuals with 7 repeats have increased risk of developing ADHD (Thapar et al., 2005). Mice engineered to lack D4 have reduced locomotor activity, increased sensitivity to drugs of abuse that elevate dopamine (cocaine, methamphetamine and ethanol), and reduced response to novelty (Rubinstein et al., 1997; Dulawa et al., 1999). Millipore's D4 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D4 interactions and its ligands. The membrane preparations exhibit a Kd of 0.86 nM for [125I]- iodospiperone. With 0.5 nM [125I]- iodospiperone, 5 μg/well D4 Membrane Prep yields greater than 6 fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human D4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_0000797
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 D4 靶子系列 Dopamine 孵化条件 : Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in assay buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Assay buffer: 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 5 mM KCl, 1.5 mM CaCl2, 4 mM MgCl2, 120 mM NaCl, filtered and stored at 4°C
Radioligand: [125I]-iodospiperone (PerkinElmer NEX284)
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 6-fold signal:background with [125I]-iodospiperone at 0.5 nM.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of D4 membrane prep. 5 µg/well Signal:Background 10.7 Specific Binding (cpm) 5996
SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 4.45 pmol/mg
Kd: 0.86 nM for [125I]-iodospiperone
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
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