merck millipore,默克密理博,HTS130RTA,Ready-to-Assay™ LPA3 Lysophospholipid Receptor Frozen Cells

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merck millipore,默克密理博,HTS130RTA,Ready-to-Assay™ LPA3 Lysophospholipid Receptor Frozen Cells
产品名称：Ready-to-Assay™ LPA3 Lysophospholipid Receptor Frozen Cells
产品型号：HTS130RTA
Human LPA3 / EDG7 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS130RTA,Ready-to-Assay™ LPA3 Lysophospholipid Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS130RTA,Ready-to-Assay™ LPA3 Lysophospholipid Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	LPA3 / EDG7

描述
产品目录编号	HTS130RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ LPA3 Lysophospholipid Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA3 is expressed primarily in testes, prostate, heart and pancreas. Mice lacking LPA3 have low expression of uterine COX-2 and display decreased uterine implantation and embryo spacing (Ye et al., 2005). Millipore’s cloned human LPA3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at LPA3.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human LPA3 / EDG7 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_012152
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	LPA3 / EDG7
靶子系列	Lysophospholipid
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.