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merck millipore,默克密理博,HTS127M,ChemiScreen™ Membrane Preparation Recombinant Human AMY1 Calcitonin Receptor
产品名称：ChemiScreen™ Membrane Preparation Recombinant Human AMY1 Calcitonin Receptor
产品型号：HTS127M
Human AMY1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS127M,ChemiScreen™ Membrane Preparation Recombinant Human AMY1 Calcitonin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS127M,ChemiScreen™ Membrane Preparation Recombinant Human AMY1 Calcitonin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 AMY1 描述 产品目录编号 HTS127M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Membrane Preparation
Recombinant Human AMY1 Calcitonin Receptor概述 Full-length human CALCR (isoform 2) and RAMP1 cDNAs encoding AMY1 背景信息 The calcitonin family of peptides, which includes calcitonin, two calcitonin-related gene peptides (CGRPs), adrenomedullin, and amylin, share a disulfide-bonded loop, an amphipathic a-helix, and an amidated C-terminus. The peptides influence a variety of physiological effects, including vascular tone, food intake and bone metabolism. The biological effects of the peptides are mediated by two class B GPCRs, the CT and CL receptors (Poyner et al., 2002). The pharmacology of the CT and CL receptors is strongly influenced by their association with a family of three receptor activity modifying proteins (RAMPs), which are 14-17 kD single pass transmembrane proteins. When expressed with no modifying proteins, the CT receptor binds with high affinity only to calcitonin. The CT receptor in complex with RAMP proteins binds amylin with high affinity, as well as calcitonin and αCGRP; the CT receptor complexes with RAMP1, RAMP2 and RAMP3 are termed AMY1, AMY2 and AMY3. Amylin (also known as islet amyloid polypeptide) is produced by pancreatic beta cells. Experiments with amylin-deficient mice indicate that amylin and its receptors control food intake and pain perception. Pramlintide, a synthetic analog of amylin, is used clinically for glucose control in diabetics. Chemicon's AMY1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of AMY1 interactions with hCGRP. The membrane preparations exhibit a Kd of 0.29 nM for [125I]-hCGRP. With 10 ug/well AMY1 Membrane Prep and 0.2 nM [125I]-hCGRP, a greater than 10-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled hCGRP at 0.2 nM. Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human AMY1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Only Entrez基因编号 - NM_001742.2
基因符号 - CRT
- CALCR
- CTR
- CTR1
- CT-R
GPCR类别 B 宿主细胞 Chem-1 蛋白靶 AMY1 靶子系列 Calcitonin UniProt编号 - P30988
UniProt汇总 FUNCTION: SwissProt: P30988 # This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
SIZE: 490 amino acids; 57303 Da
SUBUNIT: Interacts with GPRASP2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P30988 ## Belongs to the G-protein coupled receptor 2 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-hCGRP (Perkin Elmer # NEX354)
Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 SPECIFICATIONS: 1 unit = 10 μg membrane preparation
Bmax: 0.45 pmol/mg
Kd: 0.29 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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下一件merck millipore产品：merck millipore,默克密理博,521215,PDGF, AA Homodimer, Human, Recombinant, E. coli
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