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merck millipore,默克密理博,HTS121M,ChemiSCREEN™ Human Motilin Receptor Membrane Preparation
产品名称：ChemiSCREEN™ Human Motilin Receptor Membrane Preparation
产品型号：HTS121M
Human motilin receptor / MTLR / GPR38 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS121M,ChemiSCREEN™ Human Motilin Receptor Membrane Preparation
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 motilin receptor / MTLR / GPR38 描述 产品目录编号 HTS121M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Human Motilin Receptor Membrane Preparation 概述 Human Motilin Receptor 背景信息 Motilin is a 22 amino acid peptide that potently stimulates gastrointestinal contractility. The biological effects of motilin are mediated by a Gq-coupled seven transmembrane protein, currently termed motilin receptor (MR), that shares significant sequence similarity with the ghrelin receptor (Feighner et al., 1999). The motilin receptor is also activated by the antibiotic erythromycin; this interaction appears to mediate some of the gastrointestinal side effects of erythromycin. Although motilides (non-antibiotic derivatives of erythromycin) such as ABT-229 have been investigated for treatment of diabetic gastroporesis, the effectiveness has been limited by tachyphylaxis (decreased response to ligand) resulting from receptor downregulation (Thielemans et al., 2005). Agonists of the motilin receptor with reduced densensitization activity remain a potential treatment for disorders of gastric motility. Chemicon's cloned human Motilin Receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Motilin Receptor expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between the Motilin Recptor and its ligands. Chemicon's motilin membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of motilin receptor interactions with motilin. The membrane preparations exhibit a Kd of 0.43nM for [125I]-Motilin. With 5.0 µg/well Motilin Membrane Prep and 0.5 nM [125I]-Motilin, a greater than 30-fold signal-to-background ratio was obtained. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human motilin receptor / MTLR / GPR38 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_001507.1
基因符号 - MTLR1
- MLNR
- GPR38
- MTLR
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 motilin receptor / MTLR / GPR38 靶子系列 Motilin UniProt编号 - O43193
UniProt汇总 FUNCTION: SwissProt: O43193 # Receptor for motilin.
SIZE: 412 amino acids; 45344 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed only in thyroid, stomach, and bone marrow.
SIMILARITY: SwissProt: O43193 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] Motilin (Perkin Elmer # NEX378)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 30-fold signal:background with 125I-labeled motilin at 0.5 nM.产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Motilin Receptor membrane prep:
5 µg/well 10 µg/well Signal:Background 75 65 Specific Binding (cpm) 46348 54457
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 5.6 pmol/mg
Kd: 0.43 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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