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品种反应性 主要应用 宿主 格式 抗体类型 Rhesus Macaque, H IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB10511 Replaces 04-1055 描述 Anti-ATG7 Antibody Alternate Names - ubiquitin activating enzyme E1-like protein
- APG7 autophagy 7-like
- APG7 autophagy 7-like (S. cerevisiae)
- ATG7 autophagy related 7 homolog (S. cerevisiae)
- Ubiquitin-activating enzyme E1-like protein
背景信息 Autophagy is the process by which cells recycle cytoplasm and dispose of excess or defective organelles. This process is suggested to be involved in development, differentiation, growth regulation, and tissue remodeling in multicellular organisms. Autophagy-related protein 7 (ATG7) is an ubiquitin-like modifier-activating enzyme and is essential for multisubstrates such as ATG8-like proteins GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A. ATG 7 is also involved in ATG12 conjugation to ATG5 and ATG3. In this interaction, an ATG7-ATG3 complex plays a role in the conjugation of ATG12 to ATG5. Recently, ATG7 deficiency has been linked to human breast cancer resistance to anti-cancer therapies. 产品信息 格式 Affinity Purified 控制 - Human lymph node tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ATG7 Antibody detects level of ATG7 & has been published & validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected ATG7 in vascular and high grade prostate carcinoma tissues. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of human ATG7. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes ATG7 at the N-terminus. 品种反应性 Rhesus MacaqueHuman Species Reactivity Note Demonstrated to react with human.
Predicted to react with rhesus macaque, orangutan, bovine, canine, and yeast based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001129503
Entrez基因汇总 This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq]. 基因符号 - hAGP7
- APG7-LIKE
- APG7-like
- APG7L
- GSA7
纯化方法 Affinity Purified UniProt编号 - O95352
UniProt汇总 FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy By similarity.
SUBUNIT STRUCTURE:Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm Probable.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Ref.4
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12 (By similarity).
SEQUENCE SIMILARITY: Belongs to the ATG7 family.产品使用声明 质量保证 Evaluated by Western Blot in human lymph node tissue lysate.
Western Blot Analysis: 5 µg/mL of this antibody detected ATG7 in 10 µg of human lymph node tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
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