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merck millipore,默克密理博,HTS105M,ChemiSCREEN™ Human MC4 Melanocortin Receptor Membrane Preparation
产品名称：ChemiSCREEN™ Human MC4 Melanocortin Receptor Membrane Preparation
产品型号：HTS105M
Human MC4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS105M,ChemiSCREEN™ Human MC4 Melanocortin Receptor Membrane Preparation
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-3 MC4 描述 产品目录编号 HTS105M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Human MC4 Melanocortin Receptor Membrane Preparation 概述 Full-length human MC4R cDNA encoding for MC4 gene Alternate Names - MC4R
背景信息 The melanocortins, α-, β- and γ-melanocyte-stimulating hormones (MSHs), are peptides derived from a precursor protein POMC. The MSH peptides bind to a family of five Gs-coupled seven transmembrane recptors (MCRs) and play important roles in energy balance, reproductive function, pigmentation and inflammation (Gantz and Fong, 2003). The activity of the melanocortins is modulated by endogenous antagonist proteins, agouti and agouti-related protein (AGRP). MC4R is expressed primarily in the CNS and appears to play a prominent role in energy homeostasis. The hypothalamus, which is a major central site for control of feeding behavior, prominently expresses MC4R. Targeted deletion of MC4R in mice and naturally occurring mutations in MC4R in humans result in obese phenotypes. In addition, blockade of MC4R function by antagonists and targeted deletion of the gene in mice reverses melanocortin agonist-induced inhibition of food intake and promotes weight gain in uremia and cancer-induced cachexia (Cheung et al., 2005; Markison et al., 2005). Chemicon's MC4 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of MC4 interactions with αMSH. The membrane preparations exhibit a Kd of 0.71-0.77 nM for [125I]-NDP-αMSH. With 10 µg/well MC4 Membrane Prep and 0.5 nM [125I]-NDP-αMSH, a greater than 15-fold signal-to-background ratio was obtained. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human MC4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_005912.2
基因符号 - MGC126851
- MC4R
- MC4-R
- MGC138197
GPCR类别 A 宿主细胞 Chem-3 蛋白靶 MC4 靶子系列 Melanocortin UniProt编号 - P32245
UniProt汇总 FUNCTION: SwissProt: P32245 # Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
SIZE: 332 amino acids; 36943 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain, placental, and gut tissues.
DISEASE:SwissProt: P32245 # Defects in MC4R are a cause of autosomal dominant obesity [MIM:601665].
SIMILARITY: SwissProt: P32245 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C.
Radioligand: [125I] NDP-αMSH (Perkin Elmer # NEX352)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 15-fold signal:background with 125I-labeled NDP-αMSH at 0.5 nM.产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of MC4 membrane prep:
10 µg/well Signal:Background 26.7 Specific Binding (cpm) 10325
SPECIFICATIONS: 1 unit = 10 μg membrane preparation
Bmax: 5.5 pmol/mg
Kd: 0.74 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units 包装 2 mL, 1 mg/mL
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