merck millipore,默克密理博,MABE195,Anti-UBF Antibody, clone EP2741Y, rabbit monoclonal

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merck millipore,默克密理博,MABE195,Anti-UBF Antibody, clone EP2741Y, rabbit monoclonal
产品名称：Anti-UBF Antibody, clone EP2741Y, rabbit monoclonal
产品型号：MABE195
Use Anti-UBF Antibody, clone EP2741Y (Rabbit Monoclonal Antibody) validated in WB, FC, IHC(P) & ICC to detect UBF also known as Autoantigen NOR-90.

merck millipore,默克密理博,MABE195,Anti-UBF Antibody, clone EP2741Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABE195,Anti-UBF Antibody, clone EP2741Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ICC, WB, FC	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABE195
描述	Anti-UBF Antibody, clone EP2741Y, rabbit monoclonal
Alternate Names	nucleolar transcription factor 1 upstream binding transcription factor, RNA polymerase I Autoantigen NOR-90 Upstream-binding factor 1
背景信息	The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. UBF binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner (1). Experiments reveal that a cooperative interaction between UBF and SL1 leads to the formation of a new protein-DNA complex at the UCE and core elements which indicate that formation of the UBF-SL1 complex is vital for transcriptional activation by UBF (2). UBF contains six HMG box domains. The contribution of each HMG box motif to its function is different (3). Its believed that HMG motifs are the DNA-binding domains of UBF. Altering one of these boxes, as in the second form of UBF, may alter the functional characteristics of the transcription factor. Thus, the existence of different forms of UBF may have important ramifications for transcription by RNA polymerase I (4).

产品信息
格式	Diluted Culture Supernatant
控制	HeLa and A431 cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Use Anti-UBF Antibody, clone EP2741Y (Rabbit Monoclonal Antibody) validated in WB, FC, IHC(P) & ICC to detect UBF also known as Autoantigen NOR-90.
主要应用	Immunocytochemistry Western Blotting Flow Cytometry
应用说明	Immunocytochemistry Analysis: 1:100-250 dilution from a representative lot was used in IC. Flow Cytometry Analysis: 1:30 dilution from a representative lot was used in FC. Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected UBF in human breast carcinoma tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to human UBF near the N-terminus.
表位	Near N-terminus
克隆	EP2741Y
宿主	Rabbit
特异性	This antibody recognizes UBF near the N-terminus.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with human and mouse.
抗体类型	Monoclonal Antibody
基因符号	UBF1 UBF NOR-90 UBF-1
纯化方法	Unpurified
UniProt编号	P17480
UniProt汇总	FUNCTION: Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. SUBUNIT STRUCTURE: Homodimer. Interacts with TBP. Interacts with RASL11A, TAF1A and TAF1D. Binds to IRS1 and PIK3CA By similarity. SUBCELLULAR LOCATION: Nucleus › nucleolus By similarity. PTM: Phosphorylated and activated by PIK3CA By similarity. SEQUENCE SIMILARITIES: Contains 6 HMG box DNA-binding domains.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa and A431 cell lysates. Western Blot Analysis: 1:1,000-10,000 dilution of this antibody detected UBF in HeLa and A431 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.