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merck millipore,默克密理博,HTS104RTA,Ready-to-Assay™ β1 Adrenergic Receptor Frozen Cells
产品名称：Ready-to-Assay™ β1 Adrenergic Receptor Frozen Cells
产品型号：HTS104RTA
Human beta 1 GPCR frozen cells for Calcium Flux FLIPR Assays.
merck millipore,默克密理博,HTS104RTA,Ready-to-Assay™ β1 Adrenergic Receptor Frozen Cells
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Calcium Flux Assays Human Chem-1 beta 1 描述 产品目录编号 HTS104RTA 商名 - Ready-to-Assay
描述 Ready-to-Assay™ β1 Adrenergic Receptor Frozen Cells 背景信息 Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The endogenous catecholamines epinephrine and norepinephrine have profound effects on smooth muscle activity, cardiac function, carbohydrate and fat metabolism, hormone secretion, neurotransmitter release, and central nervous system actions. These activities are mediated by GPCRs belonging to two subfamilies, the α- and β-adrenergic receptors (Bylund et al., 1994). The three members of the β-adrenergic receptor family, β1, β2 and β3, couple to Gs to increase cAMP upon activation. In the heart, the β1 receptor constitutes 70-80% of the β-adrenergic receptors. Activation of cardiac β-adrenergic receptors, acutely increases heart rate, cardiac output, and cardiac automaticity, and chronically increases cardiac myocyte apoptosis. In failing hearts, the β1 subtype is downregulated and desenstitized, probably as a result of increased catecholamine levels. As a result, β-adrenergic receptor antagonists (β blockers) are effective in the treatment of congestive heart failure and arrhythmia (Lohse et al., 2003). Millipore’s cloned human β1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant β1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at β1.产品信息 部件 - Fifty (50) mL of Media Component.
- Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法 Fluorescent 应用 应用 Human beta 1 GPCR frozen cells for Calcium Flux FLIPR Assays. 应用说明 Calcium Flux Assays 生物信息 品种 Human Entrez基因编号 - NM_000684
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 beta 1 靶子系列 Adrenergic 细胞系类型 - Ready-to-Assay
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
包装信息 数量 1 kit
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