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主要应用 品种 EA Human 描述 产品目录编号 03-233 品牌系列 Upstate
商名 - Upstate
描述 HDAC3 (His-tag) human recombinant 概述 Human recombinant HDAC3 (His-tag) produced in insect cells 背景信息 Unlike other class I HDAC enzymes, HDAC3 is found in co-repressor complexes with the SMRT/N-CoR proteins. HDAC3 contains a unique domain structure which includes both nuclear localization and nuclear export sequences. HDAC3 has been implicated in cancer, as HDAC inhibitors produce anti-proliferative effects in various cancer cells. HDAC3 may also play a role in heart disease, which may involve deacetylation of MEF2. 产品信息 格式 Purified 演示 In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol 应用 应用 Human recombinant HDAC3 (His-tag) produced in insect cells. 主要应用 - Enzyme Assay
应用说明 HDAC deacetylase assays 生物信息 纯度 Purity >50% by SDS-PAGE 来源 Human HDAC3 品种 Human 特异活性 > 1U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity 物理形式 Frozen solution 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC3 into separate tubes before storing at -80°C. 包装信息 数量 50 µg
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