merck millipore,默克密理博,HTS075RTA,Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells

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merck millipore,默克密理博,HTS075RTA,Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells
产品名称：Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells
产品型号：HTS075RTA
Human M5 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS075RTA,Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS075RTA,Ready-to-Assay™ M5 Acetylcholine (Muscarinic) Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	M5

描述
产品目录编号	HTS075RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ M₅ Acetylcholine (Muscarinic) Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M5 receptor, along with the M1 and M3 receptors, signal through Gq/11 and subsequent release of Ca++ from the ER (Caulfield and Birdsall, 1998). Although M5 is expressed in the CNS at relatively low levels, it appears to be the only muscarinic acetylcholine receptor expressed in midbrain dopaminergic neurons, and it has been shown to mediate dopamine release from these neurons. The M5 receptor is also expressed in blood vessels in the brain and the periphery, and studies with M5 knockout mice demonstrate that M5 mediates the vasodilatory action of acetylcholine on cerebral microvessels. Further studies with M5 knockout mice also indicate a role for M5 in the rewarding effects of cocaine and morphine (Wess, 2004). Millipore’s cloned human M5-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant M5 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at M5.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human M5 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_012125
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	M5
靶子系列	Acetylcholine (muscarinic)
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.