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merck millipore,默克密理博,PS1089,PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb
产品名称：PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb
产品型号：PS1089
This PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).
merck millipore,默克密理博,PS1089,PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA. 产品目录编号 PS1089 品牌系列 Calbiochem®
应用数据 
Detection of human RhoA, phosphorylated at Ser188, by immunoblotting. Samples: RhoA GST fusion protein left unphosphorylated (lanes 1,3) or phosphorylated in vitro by PKA (lanes 2,4,5,6). Primary antibodies: Anti-RhoA (detecting total Rho; lanes 1,2) and PhosphoDetect™ Anti-RhoA (pSer188) (Cat. No. PS1089) (1:1000) preincubated without blocking peptide (lanes 3,4), preincubated with phosphoRhoA (pSer188) blocking peptide (lane 5), and preincubated with nonspecific phosphoserine containing blocking peptide (lane 6). Detection: chemiluminescence.参考 参考 Ellerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
Lang, P., et al. 1996. EMBO J. 15, 510.产品信息 形式 Liquid 配方 In PBS, 1 mg/ml BSA, 50% glycerol. 正控制 RhoA GST fusion protein 防腐剂 ≤0.1% sodium azide 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
生物信息 免疫原 a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Do not aliquot.
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