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品种反应性 主要应用 宿主 格式 抗体类型 R, B, H IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-474 品牌系列 Upstate
商名 - Upstate
描述 Anti-GABA A Receptor β2/3 Antibody Alternate Names - gamma-aminobutyric acid A receptor, alpha 1
- GABA(A) receptor subunit alpha-1
- gamma-aminobutyric acid (GABA) A receptor, alpha 1
- alpha 1
背景信息 The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 3 different isoforms of the beta subunit, and receptor subunit expression varies during development. Additionally, GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 and beta 2/3 subunits increase upon chronic exposure to alcohol, and may be potential therapeutic targets for alcohol dependency. The beta 2 subunit is required for receptor assembly and surface expression, while mice with an inactivated beta3 subunit exhibit epilepsy and cleft palate. 产品信息 格式 Purified 控制 - Brain tissue
演示 Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. 应用 应用 Anti-GABA A Receptor β2/3 Antibody, is an antibody against GABA A Receptor β2/3 for use in WB, IP, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunoprecipitation: 4 μg of a previous lot immunoprecipitated the GABAA Receptor β2 and β3 subunits from 500 μg of rat brain microsomal tissue extract. Due to interference of the IgG heavy chain, the immunoprecipitated GABAA receptor complex was confirmed using a polyclonal anti-GABAA receptor α1 subunit (Catalog # 06-868).
Immunohistochemistry: This antibody has been reported to detect the GABAA Receptor β2 and β3 subunits in paraffin embedded and frozen rat brain sections which had been fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100.生物信息 免疫原品种 Affinity-purified GABAA receptor isolated from bovine brain. Clone 62-3G1 克隆 clone 62-3G1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Specific for the GABAA Receptor β2 subunit, MW ~55 kDa and β3 subunit, MW ~57 kDa. 同种型 IgG 品种反应性 RatBovineHuman Species Reactivity Note Human, rat, bovine. 抗体类型 Monoclonal Antibody Entrez基因编号 - XM_612515, XM_583106
Entrez基因汇总 GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. 基因符号 - EJM
- GABRA1
- GABA
纯化方法 Protein G purfied UniProt编号 - P14867
UniProt汇总 FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 456 amino acids; 51802 Da
SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.产品使用声明 质量保证 Evaluated by western blot on rat brain microsomal preparation.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected the GABAA Receptor β2 and β3 subunits from 20 μg of rat brain microsomal preparation (Catalog # 12-144).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2°C to 8°C from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB2601,Anti-CIB1 Antibody, clone 3C5
下一件merck millipore产品：merck millipore,默克密理博,20-103,Phosphate Standard
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