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merck millipore,默克密理博,HTS070M,ChemiScreen™ Membrane Preparation Recombinant Human ETA Endothelin Receptor, Type A
产品名称：ChemiScreen™ Membrane Preparation Recombinant Human ETA Endothelin Receptor, Type A
产品型号：HTS070M
Human ETA GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS070M,ChemiScreen™ Membrane Preparation Recombinant Human ETA Endothelin Receptor, Type A
- 产品介绍
- merck millipore,默克密理博,HTS070M,ChemiScreen™ Membrane Preparation Recombinant Human ETA Endothelin Receptor, Type A
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 ETA 描述 产品目录编号 HTS070M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Membrane Preparation
Recombinant Human ETA Endothelin Receptor, Type A概述 Full-length human EDNRA cDNA encoding ETA 背景信息 The endothelin family of peptides are potent vasoconstrictors synthesized by endothelial cells in both constitutive and inducible pathways. The biological actions of endothelins are mediated by two GPCRs, ETA and ETB. ETA is the predominant receptor on smooth muscle cells and thus is the primary mediator of the vasoconstrictor activity of endothelins in vivo. Endothelin receptors are also found in the epithelium and central nervous system (Davenport, 2002). Chemicon's ETA membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of ETA interactions with its ligands. The membrane preparations exhibit a Kd of 85 pM for [125I]-Endothelin-1. With 2.5 or 10 ug/well ETA Membrane Prep and 0.35 nM [125I]-Endothelin-1, greater than 7-fold and 9-fold signal-to-background ratio were obtained, respectively. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to 0.5 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human ETA GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - S63938
Entrez基因汇总 This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. 基因符号 - EC 2.7.7.7
- POLH
- XP-V
- FLJ21978
- FLJ16395
- RAD30
- XPV
- RAD30A
- OTTHUMP00000039868
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 ETA 靶子系列 Endothelin UniProt编号 - Q9Y253
UniProt汇总 FUNCTION: SwissProt: Q9Y253 # DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.
COFACTOR: Divalent metal cations. Prefers magnesium, but can also use manganese.
SIZE: 713 amino acids; 78413 Da
SUBUNIT: Binds REV1L (By similarity). Binds monoubiquitinated PCNA, but not unmodified PCNA. Binds POLI.
SUBCELLULAR LOCATION: Nucleus. Note=Accumulates at replication forks after DNA damage.
DOMAIN: SwissProt: Q9Y253 The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
DISEASE: SwissProt: Q9Y253 # Defects in POLH are the cause of xeroderma pigmentosum variant type (XPV) [MIM:278750]; also designated as XP-V. Xeroderma pigmentosum (XP) is an autosomal recessive disease due to deficient nucleotide excision repair. It is characterized by hypersensitivity of the skin to sunlight, followed by high incidence of skin cancer and frequent neurologic abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most XPV patients do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.
SIMILARITY: Belongs to the DNA polymerase type-Y family. & Contains 1 umuC domain.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] Endothelin-1 (Perkin Elmer#:NEX-259 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled Endothelin-1 at 0.35 nM产品使用声明 质量保证 Bmax: 1.23 pmol/mg
Kd: 85 pM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 1 mL 包装 200 units
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