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品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, R, Ca, Eq WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-070 描述 Anti-ATR Antibody Alternate Names - protein kinase ATR
- Ataxia telangiectasia and Rad3-related protein
- FRAP-related protein 1
- FRAP-related protein-1
- MEC1, mitosis entry checkpoint 1, homolog
- Rad3 related protein
- ataxia telangiectasia and Rad3 related
- ataxia telangiectasia and Rad3 related protein
背景信息 ATR (Ataxia Telangiectasia and Rad3-related kinase) is a protein kinase that regulates cell cycle checkpoints and DNA repair. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1). ATR belongs to the PI3/PI4-kinase family, and is most closely related to ATM (Ataxia Telangiectasia Mutated kinase). 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit serum in 0.1M Tris-Glycine (pH7.4) and 150mM NaCl containing 0.05% sodium azide. 应用 应用 Anti-ATR Antibody detects level of ATR & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: A 1:500 dilution from a previous lot of this antibody was used to was detect ATR in HeLa, NIH3T3, and C6 cell lysates. 生物信息 免疫原品种 KLH linear peptide corresponding to amino acids 1887-1902 of ATR. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes ATR. 品种反应性 BovineHumanMouseRatCanineHorse Species Reactivity Note Proven to react with human, mouse, and rat. Predicted to react with dog, horse, and cow based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001175
Entrez基因汇总 The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. 基因符号 - SCKL1
- EC 2.7.11.1
- FRP1
- MEC1
- SCKL
纯化方法 Affinity Purfied UniProt编号 - Q13535
UniProt汇总 FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Manganese.
ENZYME REGULATION: Activated by DNA and inhibited by BCR-ABL oncogene. Slightly activated by ATRIP. Inhibited by caffeine, wortmannin and LY294002.
SUBUNIT STRUCTURE: Forms an heterodimer with ATRIP. Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164.
SUBCELLULAR LOCATION: Nucleus. Note: Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
TISSUE SPECIFICITY: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
PTM: Phosphorylated; autophosphorylates in vitro.
INVOLVEMENT IN DISEASE: Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.产品使用声明 质量保证 Evaluated by Western Blot on HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect ATR in HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
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