merck millipore,默克密理博,HTS034M,ChemiSCREEN™ NTR1 Membrane Preparation

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPgammaS binding.	Human	Chem-2	NTR1 / NTS1

描述
产品目录编号	HTS034M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ NTR1 Membrane Preparation
概述	Human NTR1
Alternate Names	Neurotensin III
背景信息	Neurotensin is a 13 amino acid peptide that induces hypothermia and mediates analgesia when administered centrally, and also mediates the behavioral effects of antipsychotic drugs in rodents (Kinkead and Nemeroff, 2004). In addition, several intestinal functions, such as contractility and inflammation, are mediated by neurotensin (Castagliuolo et al., 1999). The actions of neurotensin are mediated by two GPCRs, NTR1 (or NTS1) and NTR2 (or NTS2). NTR1 knockout mice are resistant to the hypothermic, analgesic and contractile effects of neurotensin, indicating that NTR1 is the primary mediator of these effects (Pettibone et al., 2002). Small molecule antagonists, including SR48692 and SR142948A, that are partially selective for NTR1 also interfere with the biological effects of neurotensin (Labbé-Jullié et al., 1998). Chemicon's NTR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of neurotensin/NTR1 interactions. The membrane preparations exhibit a Kd of 1.5 nM for [¹²⁵I]-neurotensin. With 2.5 μg/well NTR1 Membrane Prep and 0.5 nM [¹²⁵I]-neurotensin, a greater than 7-fold signal-to-background ratio is obtained.

产品信息
格式	Membranes
演示	PACKAGING: Packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSAPackaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80oC.

应用
应用	Human NTR1 / NTS1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
应用说明	Radioligand binding assay, and GTPgammaS binding.

生物信息
浓缩	0.5 mg/mL
品种	Human
品种反应性	Human
Entrez基因编号	NM_002531.2
Entrez基因汇总	Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion.
基因符号	NTR NTSR1 NTRR NT-R-1 NTRH
GPCR类别	A
宿主细胞	Chem-2
蛋白靶	NTR1 / NTS1
靶子系列	Neurotensin
UniProt编号	P30989
UniProt汇总	FUNCTION: SwissProt: P30989 # Receptor for the tridecapeptide neurotensin. It is associated with G proteins that activate a phosphatidylinositol- calcium second messenger system. SIZE: 418 amino acids; 46259 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P30989 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	Binding buffer: 50mM HEPES, 5mM MgCl2, 1mM CaCl2, 0.2% BSA, pH to 7.4 and filter. Radioligand: [125I] Neurotensin (PerkinElmer#: NEX198) One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled neurotensin at 0.5 nM.

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with NTR1 membrane prep:

	5 µg/well
Signal:Background	47
Specific Binding (cpm)	17556

SPECIFICATIONS: 1 unit = 5 µg
Bmax: 12.9 pmol/mg
Kd: 1.5 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

包装信息
数量	200 assays

merck millipore,默克密理博,HTS034M,ChemiSCREEN™ NTR1 Membrane Preparation

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