- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
描述 产品目录编号 14-352 品牌系列 Upstate
商名 - Upstate
描述 Raf-1 Protein, Active, 10 µg 概述 N-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D and Y341D 应用 应用 Active, N-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D & Y341D, for use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 Expressed by baculovirus in Sf21 insect cells 品种 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_002880.2
Entrez基因汇总 Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text] 基因符号 - RAF
- RAF1
- C-RAF
- cRaf
- Raf-1
- c-Raf
- CRAF
蛋白靶 c-RAF 纯化方法 Glutathione agarose affinity chromatography 靶子系列 TKL UniProt编号 - P04049
UniProt汇总 FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.产品使用声明 质量保证 routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -70°C 包装信息 数量 10 µg 包装 Also available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.
上一件merck millipore产品：merck millipore,默克密理博,1171,Adenosine 5ʹ-Diphosphate, Monosodium Salt - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,196871,InSolution™ BAY 11-7082 - Calbiochem
相关产品
merck millipore,默克密理博,ABE212,Anti-Set1A Antibody
merck millipore,默克密理博,ECM532,α/β Integrin-mediated Cell Adhesion Array Combo Kit, colorimetic
merck millipore,默克密理博,ABE386,Anti-Flightless-1 homolog Antibody
merck millipore,默克密理博,1171,Adenosine 5ʹ-Diphosphate, Monosodium Salt - Calbiochem
merck millipore,默克密理博,196871,InSolution™ BAY 11-7082 - Calbiochem
merck millipore,默克密理博,650212,ProteoExtract All-in-One Trypsin Digestion Kit
merck millipore,默克密理博,69770,pET-21(+) DNA - Novagen
merck millipore,默克密理博,HTS034M,ChemiSCREEN™ NTR1 Membrane Preparation
