merck millipore,默克密理博,AB5744,Anti-Nkx-5.1 Antibody

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merck millipore,默克密理博,AB5744,Anti-Nkx-5.1 Antibody
产品名称：Anti-Nkx-5.1 Antibody
产品型号：AB5744
This Anti-Nkx-5.1 Antibody is validated for use in WB, IH for the detection of Nkx-5.1.

merck millipore,默克密理博,AB5744,Anti-Nkx-5.1 Antibody

产品介绍

merck millipore,默克密理博,AB5744,Anti-Nkx-5.1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5744
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Nkx-5.1 Antibody
Alternate Names	NK-2

产品信息
格式	Affinity Purified
演示	Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	This Anti-Nkx-5.1 Antibody is validated for use in WB, IH for the detection of Nkx-5.1.
主要应用	Immunohistochemistry Western Blotting
应用说明	Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide, amino acids 10-23 from mouse Nkx5.1.
宿主	Rabbit
特异性	Recognizes Nkx5.1, (NK-2 family), a homeodomain transcription factor.
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_001105574.1
基因符号	NKX5-1 HMX3 Nkx5-1 HCG1781691 ORFNames=hCG_1781691
UniProt编号	A8MU06
UniProt汇总	SIZE: 323 amino acids; 34900 Da

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

储存和货运信息

存储条件

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

包装信息
数量	100 µg

merck millipore,默克密理博,AB5744,Anti-Nkx-5.1 Antibody

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