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merck millipore,默克密理博,ABE212,Anti-Set1A Antibody
产品名称：Anti-Set1A Antibody
产品型号：ABE212
Use Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1.
merck millipore,默克密理博,ABE212,Anti-Set1A Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ChIP, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 ABE212 描述 Anti-Set1A Antibody Alternate Names - SET domain-containing protein 1A
- SET domain containing 1A
- Lysine N-methyltransferase 2F
- histone-lysine N-methyltransferase SETD1A
- Set1/Ash2 histone methyltransferase complex subunit SET1
背景信息 Set1A is a histone methyltransferase (HMT) that is part of the SET1 HMT complex. This protein specifically methylates lysine 4 of histone H3 if the adjacent lysine at position 9 is also unmethylated. Studies demonstrate that Set1A and Set1B localize to largely non-overlapping regions of the chromosome. This suggests Set1A and Set1B bind to unique sets of target genes and that each protien makes non-redundant contributions to the epigenetic control of chromatin structure and gene expression. 产品信息 格式 Serum 控制 - PC3 cell lysate
演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Use Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation (ChIP):
A representative lot of this antibody was reported to work in ChIP. See Lee, J.H., et al.(2008). Mol Cell Biol. 28(2):609–618.
Immunocytochemistry:
A representative lot of this antibody was reported to work in immunocytochemistry. See . Lee, J.H., et al. (2007). J Biol Chem. 282(18):13419-13428.
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human Set1A. 宿主 Rabbit 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_055527
Entrez基因汇总 SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). 基因符号 - Set1A
- hSET1A
- SET1
- SET1A
- SETD1A
- Set1
纯化方法 Unpurified UniProt编号 - O15047
UniProt汇总 FUNCTION: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
SUBUNIT STRUCTURE: Interacts with HCFC1. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2/ASH2L and CXXC1/CFP1.
SUBCELLULAR LOCATION: Nucleus speckle. Chromosome. Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
SEQUENCE SIMILARITIES: Contains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
SEQUENCE CAUTION: The sequence AAH27450.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH35795.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
The sequence BAA20797.2 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in PC3 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Set1A on 10 µg of PC3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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