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应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPgammaS binding. Human Chem-1 CRF1 描述 产品目录编号 HTS023M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ CRF1 Membrane Preparation 概述 Full-length Human CRF1 背景信息 The CRF1 receptor is a Gs-coupled GPCR expressed in the brain and pituitary gland that binds to several neuropeptides, including corticotropin-releasing factor (CRF) and urocortin, and the amphibian peptide sauvagine (Chen et al., 1993; Dautzenberg and Hauger, 2002; Bale and Vale, 2004). CRF plays a predominant role in stress response mediated by the hypothalamic-pituitary-adrenal axis, and alterations in CRF and its receptors CRF1 and CRF2 appear to be linked to depression and anxiety (Holsboer, 1999; Bale and Vale, 2004). A number of small molecule antagonists of the CRF1 receptor have been characterized, including R121919, SC241, NBI27914, antalarmin, DMP-696, and CP 154,526. When delivered in animal models of psychiatric disorders, these antagonists display effectiveness in reducing stress-related behaviors (Kehne and De Lombaert, 2002). Chemicon's CRF1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CRF1 interactions with its ligands. The membrane preparations exhibit a Kd of 1 nM for [125I]-sauvagine. With 2.5 μg/well CRF1 Membrane Prep and 0.5 nM [125I]-sauvagine, a greater than 12-fold signal-to-background ratio is obtained. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human CRF1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding. 应用说明 Radioligand binding assay, and GTPgammaS binding. 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_004382.3
Entrez基因汇总 The corticotropin-releasing hormone receptor binds to corticotropin-releasing hormone (MIM 122560), a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress.[supplied by OMIM] 基因符号 - CRH-R1h
- CRHR1
- CRF1
- CRFR
- CRHR1f
- CRHR
- CRFR1
- CRF-R
GPCR类别 B 宿主细胞 Chem-1 蛋白靶 CRF1 靶子系列 CRF Receptor UniProt编号 - P34998
UniProt汇总 FUNCTION: SwissProt: P34998 # This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 444 amino acids; 50719 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
PTM: C-terminal Ser or Thr residues may be phosphorylated.
SIMILARITY: SwissProt: P34998 ## Belongs to the G-protein coupled receptor 2 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate (Costar 3605), and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°CRadioligand: [125I] sauvagine (Perkin Elmer#: NEX306)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled Sauvagine at 0.50 nM产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with CRF1 membrane preparation.
5 µg/well Signal:Background 13.2 Specific Binding (cpm) 31785
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 assays
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