merck millipore,默克密理博,HTS021RTA,Ready-to-Assay™ MC2 Melanocortin Receptor Frozen Cells

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merck millipore,默克密理博,HTS021RTA,Ready-to-Assay™ MC2 Melanocortin Receptor Frozen Cells
产品名称：Ready-to-Assay™ MC2 Melanocortin Receptor Frozen Cells
产品型号：HTS021RTA
Human MC2 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS021RTA,Ready-to-Assay™ MC2 Melanocortin Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS021RTA,Ready-to-Assay™ MC2 Melanocortin Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	MC2

描述
产品目录编号	HTS021RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ MC₂ Melanocortin Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The melanocortins, α-, β- and γ-melanocyte-stimulating hormones (MSHs) and adrenocorticotropin (ACTH), are peptides derived from a precursor protein POMC. The MSH peptides and ACTH bind to a family of five Class 1 Gs-coupled seven transmembrane receptors (MC1-5) and play important roles in energy balance, reproductive function, pigmentation and inflammation (Gantz and Fong, 2003). MC2, the ACTH receptor, is a member of this family but is the only one that does not bind the MSHs, it instead binds ACTH exclusively. MC2 is expressed mainly in cells of the adrenal cortex, where it signals cells in the adrenal cortex to synthesize and secrete glucocorticoids (Clark A. et al., 2006). Mutations in MC2 lead to familial glucocorticoid deficiency, or ACTH insensitivity. Familial glucocorticoid deficiency can lead to increased pigmentation and increased longitudinal bone growth (Clark A, et al. 1993, Imamine H, et al. 2005). In addition to mutations in MC2 leading to Familial glucocorticod deficiency, it has also been shown that mutations in a protein called MC2-R accessory protein (MRAP) also lead to this disorder (Clark A, 2005). Expression of MC2 on the cell surface has been found to be dependent on the interaction of MC2 with MRAP. This interaction helps to move MC2 from the endoplasmic reticulum to the cell surface. Millipore’s cloned human MC2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MC2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MC2.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human MC2 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_000529
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	MC2
靶子系列	Melanocortin
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.