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merck millipore,默克密理博,04-893,Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal
产品名称：Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal
产品型号：04-893
Detect JNK3/SAPK1b using this Anti-JNK3/SAPK1b Antibody, clone C05T validated for use in WB.
merck millipore,默克密理博,04-893,Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-893 Replaces 05-893 描述 Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal Alternate Names - stress activated protein kinase beta
- JNK3 alpha protein kinase
- MAP kinase
- MAP kinase p49 3F12
- Stress-activated protein kinase JNK3
- c-Jun N-terminal kinase 3
- c-Jun kinase 3
- mitogen-activated protein kinase 10
- stress activated protein kinase JNK3
背景信息 The stress-activated protein kinase 1 family is also referred to as the jun N-terminal kinase family, in light of the substrate preference of these serine/threonine kinases. The SAPK family shares 42-45% identity in the kinase domains with the MAPK family, and the SAPKs are activated by phosphorylation of a threonine and tyrosine by MKK4 and SKK4, just as the MAP kinases are phosphorylated on a threonine and tyrosine by MEK1 and MEK2. By contrast to the mitogen-activated kinases, the stress-activated kinases are only weakly activated by mitogenic stimuli, but potently activated by stress stimuli, such as inflammatory cytokines, ischemia, chemotherapeutic agents and irradiation.
The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.
产品信息 格式 Culture Supernatant 控制 - RIPA cell lysates from A431 cells.
演示 Cultured supernantant in 0.05% sodium azide. 应用 应用 Detect JNK3/SAPK1b using this Anti-JNK3/SAPK1b Antibody, clone C05T validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A 1:500-1:2,000 dilution of this lot detected JNK3 in RIPA lysates from A431 cells. 生物信息 免疫原品种 GST fusion protein corresponding to full-length rat JNK3/ SAPK1b 克隆 C05T 宿主 Rabbit 特异性 Recognizes JNK3/SAPK1b. Does not cross react with JNK1 or JNK2. 同种型 IgG 品种反应性 RatHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002753.2
Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
基因符号 - p54bSAPK
- EC 2.7.11.24
- FLJ12099
- FLJ33785
- JNK3
- JNK3A
- MGC50974
- OTTHUMP00000161180
- OTTHUMP00000161182
- OTTHUMP00000161183
- PRKM10
- p493F12
UniProt编号 - P49187
UniProt汇总 FUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity By similarity.
Catalytic activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Magnesium By similarity.
Enzyme regulation Activated by threonine and tyrosine phosphorylation by two dual specificity kinases, MAP2K4 and MAP2K7. MAP2K7 phosphorylates MAPK10 on Thr-221 causing a conformational change and a large increase in Vmax for the enzyme. MAP2K4 then phosphorylates Tyr-223 resulting in a further increase in Vmax. Inhibited by dual specificity phosphatases, such as DUSP1. Inhibited by HDAC9 By similarity.
SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with HDAC9 and MAPKBP1 By similarity.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM:Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme By similarity.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
产品使用声明 质量保证 Routinely evaluated by western blot on RIPA lysates from A431 cells.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. 包装信息 数量 100 µL
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