merck millipore,默克密理博,HTS004LT,ChemiBrite™ CXCR4 Chemokine Family Receptor Frozen Cells

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merck millipore,默克密理博,HTS004LT,ChemiBrite™ CXCR4 Chemokine Family Receptor Frozen Cells
产品名称：ChemiBrite™ CXCR4 Chemokine Family Receptor Frozen Cells
产品型号：HTS004LT
Human CXCR4 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS004LT,ChemiBrite™ CXCR4 Chemokine Family Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS004LT,ChemiBrite™ CXCR4 Chemokine Family Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	HEKT	CXCR4

描述
产品目录编号	HTS004LT
商名	ChemiBrite
描述	ChemiBrite™ CXCR4 Chemokine Family Receptor Frozen Cells
背景信息	Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds. Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The chemokine SDF-1α and its GPCR receptor CXCR4 have a one-to-one specificity that is unique among chemokines and their receptors. SDF-1α binds to CXCR4 expressed on hematopoietic and lymphopoietic cells, and directs their trafficking to and retention in hemato-and lymphatopoietic organs and sites of inflammation (Kucia et al., 2004). CXCR4 is expressed on several tumor cell lineages, and might be responsible for metastasis to sites of SDF-1α expression, such as bone and lymph nodes (Muller et al., 2001). In addition, CXCR4 is a coreceptor for the HIV envelope glycoprotein gp120 (Feng et al., 1996). Small molecule antagonists of CXCR4 have been developed and shown to inhibit infectivity of T-tropic HIV strains and to impair growth of brain tumors (Arakaki et al., 1999; Rubin et al., 2003). Millipore’s cloned human CXCR4 receptor-expressing ChemiBrite cells are made by transient transfection of HEK293T cells of optimized quantities of plasmids encoding the receptor, clytin and a promiscuous G protein to couple the receptor to the calcium signaling pathway and then cryopreserving the cells at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis of agonists, antagonists and modulators at the CXCR4 receptor.

产品信息
部件	Vial contains 1 ml of mycoplasma-free cells
检测方法	Chemiluminescence

应用
应用	Human CXCR4 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	M99293
GPCR类别	A
宿主细胞	HEKT
蛋白靶	CXCR4
靶子系列	Chemokine

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.