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  • merck millipore,默克密理博,FCCS025132,FlowCellect™ p38 Stress Pathway Activation Detection Kit

  • merck millipore,默克密理博,FCCS025132,FlowCellect™ p38 Stress Pathway Activation Detection Kit

    产品名称：FlowCellect™ p38 Stress Pathway Activation Detection Kit
    产品型号：FCCS025132
    This FlowCellect p38 Stress Pathway Activation Detection Kit for Flow Cytometry is designed to enable a researcher a quick & easy way to detect the phosphorylation state of P38α & ATF2 by flow cytomet More>>

    merck millipore,默克密理博,FCCS025132,FlowCellect™ p38 Stress Pathway Activation Detection Kit

  • 产品介绍
  • merck millipore,默克密理博,FCCS025132,FlowCellect™ p38 Stress Pathway Activation Detection Kit

    描述
    产品目录编号 FCCS025132
    商名
    • FlowCellect
    描述 FlowCellect™ p38 Stress Pathway Activation Detection Kit
    概述 Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of P38α and ATF2 by flow cytometry. Millipore’s FlowCellect™ p38 Stress Pathway Activation Kit was developed and tested using the activating reagent, anisomycin, in Jurkat cells but the kit can be used with other human cell lines to determine the effect of stress stimuli and chemical reagents that can induce P38 pathway signaling.
    Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit contains sufficient reagents for 25 2-color tests. The kit includes two optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
    Alternate Names
    • Flow Cellect
    背景信息 p38 is a member of the mitogen activated kinase family that responds to stress stimuli, such as inflammatory cytokines, ultraviolet irradiation, heat shock, and osmotic shock. p38 is involved in cell differentiation and apoptosis. More recently, P38 has been shown to have a role in cell cycle check points and DNA damage pathways. The upstream kinases, MKK3, MKK6 and SEK activate p38α by phosphorylation at Thr180 and Tyr182.
    Once activated, p38α MAP kinase has been shown to phosphorylate and activate a number of proteins including MAPKAP kinase 2 and the transcription factors ATF-2, MEF2 and MSK1.
    产品信息
    部件
    • 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
    • 1. 20X Anti-pP38(T180/Y182) AlexaFluor®488 Antibody: (Part No. CS205783) 1 vial containing µL.
    • 2. 20X Anti-pATF2(T69/71) AlexaFluor®647 Antibody: (Part No.CS205784) 1 vial containing 150 µL.
    • 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
    • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
    • 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
    检测方法 Fluorescent
    应用
    应用 This FlowCellect p38 Stress Pathway Activation Detection Kit for Flow Cytometry is designed to enable a researcher a quick & easy way to detect the phosphorylation state of P38α & ATF2 by flow cytometry.
    主要应用
    • Flow Cytometry
    生物信息
    宿主 Mouse
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    产品使用声明
    质量保证 Lot to lot in-process testing on all kit components
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
    储存和货运信息
    存储条件 4-8°C for antibodies and buffers
    包装信息
    数量 25 Tests / Kit

    merck millipore,默克密理博,FCCS025132,FlowCellect™ p38 Stress Pathway Activation Detection Kit

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