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检测方法 Colorimetric 描述 概述 An ELISA-based activity assay for measuring the kinase activity of purified or mTOR immunoprecipitated from cell lysates. Useful for in vitro mTOR inhibitor screening and for assessing the regulation of mTOR cell signaling. This assay utilizes a p70S6K GST fusion protein as a specific mTOR substrate. 产品目录编号 CBA055 品牌系列 Calbiochem®
参考 参考 Kong, D. and Yamori, T. 2007. Cancer Sci., 98, 1638.
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Chiang G.G. and Abraham R.T. 2005. J. Biol. Chem. 280, 25485.
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Sarbassov, D.D., et al. 2005. Science 307, 1098.
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Kimura, N., et al. 2003. Genes Cells 8, 65.
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Sekulic, A. et al. 2000. Cancer Res. 60, 3504.
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Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
Freeman, K. and Livi, G.P. 1996. Gene 172, 143.
Zheng, X.F., et al. 1995. Cell 82, 121.
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Heitman, J., et al. 1991. Science 253, 905.产品信息 检测方法 Colorimetric 声明 Sold under license of U.S. Patent 7,135,298. 形式 96 Tests 格式 96-well plate 试剂盒包含 Glutathione-Coated 96-Well Plate, mTOR Substrate, Anti-p70S6K-T389, mTOR Standard, 2X Kinase Assay Buffer, ATP Solution, 100 mM DTT, Wortmannin 50X, Kinase Stop Solution, 10X Plate Wash Concentrate, Antibody Diluent, HRP-Antibody Conjugate, TMB Substrate, ELISA Stop Solution, Plate Sealers and a user protocol. 生物信息 样品类型 Purified or partially purified enzyme 产品使用声明 预定用途 The K-LISA™ mTOR Activity Kit is suitable for measuring the kinase activity of purified or partially purified preparations of mTOR or mTOR that has been immunoprecipitated from crude cell lysates. The assay is also useful for in vitro mTOR inhibitor screening and for assessing the regulation of mTOR in cell signaling. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 ≤ -70°C
存储条件 Upon arrival store the Glutathione-Coated 96-Well Plate, the Kinase Stop Solution, and the Anti-p70S6K-T389 at 4°C; store the mTOR Standard, mTOR Substrate, 100 mM DTT, and 20 mM ATP Solution at -70°C; store the remaining components of the kit at -20°C.
Note: THE KIT IS SHIPPED ON DRY ICE, BUT SOME COMPONENTS ARE STORED AT 4°C FOR LONG-TERM STORAGE. DO NOT FREEZE/THAW THE ANTI-p70S6K-T389 ANTIBODY避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Glutathione-Coated 96-Well Plate, mTOR Substrate, Anti-p70S6K-T389, mTOR Standard, 2X Kinase Assay Buffer, ATP Solution, 100 mM DTT, Wortmannin 50X, Kinase Stop Solution, 10X Plate Wash Concentrate, Antibody Diluent, HRP-Antibody Conjugate, TMB Substrate, ELISA Stop Solution, Plate Sealers and a user protocol.
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