merck millipore,默克密理愽
  • merck millipore,默克密理博,APT129,Caspase 8 Colorimetric Activity Assay Kit 25, IETD

  • merck millipore,默克密理博,APT129,Caspase 8 Colorimetric Activity Assay Kit 25, IETD

    产品名称：Caspase 8 Colorimetric Activity Assay Kit 25, IETD
    产品型号：APT129
    Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.

    merck millipore,默克密理博,APT129,Caspase 8 Colorimetric Activity Assay Kit 25, IETD

  • 产品介绍
  • merck millipore,默克密理博,APT129,Caspase 8 Colorimetric Activity Assay Kit 25, IETD

    重要规格表

    品种反应性 主要应用 检测方法
    MaACTChromogenic
    描述
    产品目录编号 APT129
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Caspase 8 Colorimetric Activity Assay Kit 25, IETD
    概述 Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
    Alternate Names
    • FLICE
    产品信息
    部件
    • pNA Standard (Part No. 90085): 250 mL of 10 mM in DMSO
    • 5X Cell Lysis Buffer (Part No. 90065): 5 mL
    • 5X Assay Buffer (Part No. 90066): 10 mL
    • Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 mL of 2.5 mg/mL solution
    • Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 mL of 100 mM (0.05 mg/mL) in DMSO
    检测方法 Chromogenic
    应用
    应用 Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
    主要应用
    • Activity Assay
    生物信息
    品种反应性 Mammals
    Entrez基因编号
    • NM_033358.3
    • NM_001080124.1
    • NM_001080125.1
    • NM_001228.4
    • NM_033355.3
    • NM_033356.3
    Entrez基因汇总 This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
    基因符号
    • Caspase-8 subunit p10].
    • CASP8
    • MGC78473
    • CASP-8
    • MACH
    • procaspase-8
    • MCH5
    • ALPS2B
    • FLICE
    • CAP4
    • EC 3.4.22.61 [Contains: Caspase-8 subunit p18
    UniProt编号
    • Q14790
    UniProt汇总 FUNCTION: SwissProt: Q14790 # Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoforms 5, 6, 7 and 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
    SIZE: 479 amino acids; 55391 Da
    SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1.
    SUBCELLULAR LOCATION: Cytoplasm.
    TISSUE SPECIFICITY: Isoforms 1, 5 and 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus, and liver. Barely detectable in brain, testis, and skeletal muscle.DOMAIN:SwissProt: Q14790 Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
    PTM: Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. & Phosphorylated upon DNA damage, probably by ATM or ATR.
    DISEASE: SwissProt: Q14790 # Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
    SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store kit materials at -20°C up to their expiration date.

    Special Precautions:

    · After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.

    · The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
    包装信息
    数量 25 assays

    merck millipore,默克密理博,APT129,Caspase 8 Colorimetric Activity Assay Kit 25, IETD

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